Code | CSB-RA042122A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
To generate a recombinant monoclonal antibody specific to ARG1, the initial step involved immunizing a rabbit with a synthesized peptide derived from human ARG1 protein. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template to extend ARG1 antibody genes using degenerate primers. These engineered ARG1 antibody genes were incorporated into a plasmid vector and transfected into host cells for expression. The resulting ARG1 recombinant monoclonal antibody was isolated from the cell culture supernatant through affinity chromatography and evaluated for its utility in ELISA and IHC applications. It is only reactive with human ARG1 protein.
The main function of the ARG1 protein is to catalyze the conversion of arginine, an amino acid, into ornithine and urea in the urea cycle. This enzymatic reaction takes place in the liver and is a critical step in the process of removing toxic ammonia from the body. Mutations or deficiencies in the ARG1 gene can lead to a rare genetic disorder known as arginase deficiency or argininemia.
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