Product Details

Uniprot No.

Target Names

Alternative Names

arnT antibody; ARNT protein antibody; ARNT_HUMAN antibody; Aryl hydrocarbon receptor nuclear translocator antibody; bHLHe2 antibody; Class E basic helix-loop-helix protein 2 antibody; Dioxin receptor antibody; Dioxin receptor nuclear translocator antibody; Drnt antibody; HIF 1 beta antibody; HIF 1beta antibody; HIF-1-beta antibody; HIF1-beta antibody; HIF1B antibody; HIF1beta antibody; Hypoxia Inducible Factor 1 antibody; Hypoxia inducible factor 1 beta antibody; Hypoxia-inducible factor 1-beta antibody; nuclear translocator antibody; Tango antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ARNT

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2F11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ARNT, also known as HIF-1-beta, serves as an essential transcriptional partner in cellular responses to hypoxia and environmental toxins. As the obligate dimerization partner for hypoxia-inducible factors and the aryl hydrocarbon receptor, ARNT orchestrates gene expression programs critical to angiogenesis, metabolic adaptation, and xenobiotic metabolism. This central role in oxygen-sensing pathways makes ARNT a compelling target for cardiovascular research and studies exploring tumor microenvironment biology.

This recombinant monoclonal antibody, clone 2F11, offers the reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human ARNT and produced using recombinant technology, this antibody delivers sequence-defined consistency across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides strong signal amplification while maintaining specificity for your target.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms robust detection of ARNT at the expected 87 kDa molecular weight across diverse human cell lines including HeLa, MCF-7, HepG2, and K562, with recommended dilutions ranging from 1:500 to 1:5000. Immunohistochemistry performance has been validated in paraffin-embedded human prostate cancer tissue using citrate buffer antigen retrieval, with effective staining at 1:50 to 1:200 dilutions. Immunoprecipitation capability has been confirmed in HeLa whole cell lysates, enabling protein interaction studies and complex isolation experiments.

Whether investigating hypoxic signaling in cardiovascular disease models or examining ARNT's role in cellular stress responses, this antibody provides the validated performance and lot-to-lot reliability essential for generating publication-quality data.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia. The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.

Gene References into Functions

Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF1A and HIF1B binding to a MYC enhancer. PMID: 27774982
in transcriptionally active heterodimer with AHR, which recognizes the dioxin response element (DRE) in the promoter of downstream genes, right next to the DRE-docking sites is the extensive dimerization surface that is more hydrophobic than other surface areas, indicating that the dimerization interface of the AHR transcription complex is largely dictated by hydrophobic contacts PMID: 28396409
Here the authors examined the crystal structures of multi-domain NPAS1-ARNT and NPAS3-ARNT-DNA complexes, discovering each to contain four putative ligand-binding pockets. PMID: 27782878
Findings reveal that certain blood cancers rely on ARNT isoform 1 to potentiate proliferation by antagonizing RelB and p53-dependent cell cycle arrest and apoptosis. PMID: 26909609
The structure clearly disclosed that AhRR competitively represses AhR binding to ARNT and target DNA and further suggested the existence of an AhRR-ARNT-specific repression mechanism. This study provides a structural basis for understanding the mechanism by which AhRR represses AhR-mediated gene transcription. PMID: 28904176
Study showed that polymorphism rs2228099 of the ARNT gene could be a novel susceptibility gene to essential hypertension. PMID: 27977510
The results revealed a HIF-1alpha-dependent mechanism leading to ARNT upregulation in hypoxia. PMID: 27362802
The protein levels of phosphorylated (p)Akt, Erk1/2, pErk1/2, HIF1alpha and HIF1beta were significantly increased by 5.89, 0.5, 0.59, 1.46 and 0.92fold, respectively, in the patients with PAH, compared with those in the controls group PMID: 27667582
this study shows that ARNT is upregulated in skin from atopic dermatitis patients in China PMID: 27129092
Authors report here that not only HIF1alpha but also ARNT regulates VEGF expression in 3D cancer spheroids. Results suggest the utility of the in vitro 3D cancer spheroid model for investigating angiogenesis in cancerous tissues. PMID: 27542820
The results of this study indicate that ARNT depletion renders tumour cells susceptible to radiation whereas overexpression of this transcription factor confers radioresistance PMID: 26572229
HIF-1beta might act as a novel cross-link between the HIF and NF-kappaB pathways in suppression of angiogenesis by LDL, while proteasome inhibitors might promote angiogenesis by reactivating this signaling cascade under hyperlipidemia. PMID: 26388611
ARNT Val189Val polymorphism is not associated with endometriosis in Asians. PMID: 26101050
HIF1 regulates claudin-1 in intestinal epithelium. This regulation is important for intestinal epithelial tight junction integrity. PMID: 25904334
Down-regulation of ARNT promotes colorectal cancer metastasis by activating the fibronectin/integrin beta1/FAK axis. PMID: 25839165
AhR/ARNT activation and overexpression of BCL6 are collectively responsible for differential expression of more than 100 genes in diffuse large B-cell lymphoma cell line. PMID: 25769544
Data indicate that silencing of hypoxia-inducible factor-1 beta (HIF-1beta) sensitizes tumor cells to hypoxic apoptosis. PMID: 25068796
ARNT expression in the placental vasculature mediates key angiogenic expression and fetoplacental endothalial cell angiogenesis, and low ARNT expression in FGRadv ECs appears to be a key factor in deficient angiogenesis. PMID: 25343232
findings suggest that TACC3 could be recruited as a bridge to cooperatively mediate between the HIF-2alpha PAS-B.ARNT PAS-B complex, thereby participating more directly in HIF-dependent gene transcription than previously anticipated PMID: 25627682
The objective of this review is therefore to highlight and summarize current knowledge regarding the hypoxia-dependent upregulation of ARNT PMID: 24849811
Arnt suppressed CXCL8, but did not prevent the p65-activation directly. PMID: 25201625
knockdown of ARNT in cancer cells reduced the proliferation rate and the transformation ability of those cells. PMID: 24921657
Myeloid ARNT is important for innate immune function and wound healing. PMID: 24990649
We demonstrate that Arnt is an interaction partner for Miz-1, and that Arnt has a functional role in the regulation of CDKN2B PMID: 24618291
Here we discuss recent findings from our lab and others that define roles of the HIF-VEGF axis in the retina. PMID: 24664708
ARNT gene deletion is associated with chronic lymphocytic leukemia. PMID: 24249660
These findings highlight the importance of the hypoxia-sensing pathway and HIFs in clinical hematology. PMID: 24371328
Understanding the DNA binding-independent functions of ARNT may identify novel therapeutic options for the treatment of a large spectrum of disease states. PMID: 23116263
ARNT may modulate reactive oxygen species signaling and drug response in acute myeloid leukemia. PMID: 24220583
ARNT was found to be responsible for establishing the drug-resistant properties of cancer cells by upregulation of MDR1. PMID: 23907215
results suggest that polymorphism located in the promoter of the AHR gene is associated with a protective effect on vitiligo in Han Chinese populations PMID: 22211302
In loco synthesis of these highly potent AhR inducers by Malassezia yeasts could have a significant impact on skin homeostatic mechanisms and disease development. PMID: 23448877
results show that ER-beta suppresses HIF-1alpha-mediated transcription via aryl hydrocarbon receptor nuclear translocator (ARNT) downregulation, which may account for the tumour suppressive function of ER-beta PMID: 21435239
Among a panel of five different cell lines, in 518A2 cells exposed to hypoxia-mimetic cobalt chloride, HIF-1beta is rapidly elevated on protein level. PMID: 23541582
ARNT controls AREG expression and the downstream EGFR-ERK pathway in keratinocytes, at least in part, by modulating HDAC activity. PMID: 22505606
Binding of the ERalpha and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-transcription. PMID: 22328528
Ainp1 reduces the interaction between ARNT and HIF-1alpha, suppresses the formation of the HIF-1 gel shift complex, and suppresses the ARNT recruitment to the vegf promoter. PMID: 22306343
miR-24 negatively regulates ARNT expression in human liver, affecting the expression of its downstream genes. miR-24 would be one of the factors underlying the mechanisms by which ARNT protein is decreased by reactive oxygen species PMID: 22387692
Data show that ARNT is an important regulator of hepatocellular carcinoma (HCC) growth and metastasis and could be a promising prognostic candidate in HCC patients. PMID: 21544813
The presence of five unique SNPs at the ARNT locus are shown. PMID: 21828933
ARNT may regulate many processes relevant to the biology of age related macular degeneration. PMID: 22183315
mutations on ARNT PAS-B modulate coactivator selectivity and target gene induction by HIF in vivo, demonstrating a bifunctional role for transcriptional regulation by PAS domains within bHLH-PAS transcription factors PMID: 21512126
NF-kB controls the levels of HIF-1a and HIF-1b genes by direct regulation. PMID: 21298084
Cloning and functional analysis of the HIF-1beta promoter identifies a prominent region for interferon (IFN)-gamma-dependent repression. PMID: 21199896
These results reveal a novel mechanism by which ARNT acts as a modulator to bridge the c-Jun/Sp1 interaction and plays a role in EGF-mediated gene expression under normoxic conditions. PMID: 20508969
Reverse transcriptase-polymerase chain reaction analysis disclosed the existence of a ETV6-ARNT fusion gene in a patient with childhood T lymphoblastic leukemia. PMID: 20804916
the expression of miR-101 is also modulated at different physiological conditions, such as androgen stimulation and HIF-1alpha/HIF-1beta induction. PMID: 20478051
the expression of ARNT protein is significantly reduced in aryl hydrocarbon receptor interacting protein mutation+ tumors PMID: 19850893
SRC-1, NCoA-2, and p/CIP are capable of independently enhancing TCDD-dependent induction of a luciferase reporter gene by the AHR/ARNT dimer PMID: 12024042
modification by SUMO-1 chiefly at Lys(245) within the PAS domain of this protein, both in vivo and in vitro PMID: 12354770

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Subcellular Location

Nucleus.

Database Links

HGNC: 700

OMIM: 126110

KEGG: hsa:405

STRING: 9606.ENSP00000351407

UniGene: Hs.632446

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Code	CSB-RA002121A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: ARNT antibody at 1.83μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 87, 85, 86 KDa Observed band size: 87 KDa IHC image of CSB-RA002121A0HU diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating HIF-1 beta in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA002121A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA002121A0HU (3μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg)
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ARNT Recombinant Monoclonal Antibody

Product Details

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Target Background