Code | CSB-RA175460A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The ATP7B recombinant monoclonal antibody is generated through in vitro processes using synthetic genes. This methodology involves the retrieval of ATP7B antibody genes from B cells sourced from immunoreactive rabbits, followed by their amplification and cloning into appropriate phage vectors. These vectors are then introduced into mammalian cell lines, enabling the production of functional antibodies in substantial quantities. Subsequently, the ATP7B recombinant monoclonal antibody is purified from the culture supernatant of the transfected cell lines through affinity chromatography. It is well-suited for a wide range of applications, including ELISA, IHC, IF, and FC, facilitating the detection of human ATP7B protein.
ATP7B is a transmembrane copper-transporting protein that is crucial for copper homeostasis. The main role of the ATP7B protein is to transport and regulate copper within the body, primarily in the liver. Mutations in the ATP7B gene can lead to Wilson's disease.
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