BNIP3L Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA310122A0HU
Size	US$210
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Image	IHC image of CSB-RA310122A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA310122A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA310122A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

O60238

Target Names

BNIP3L

Alternative Names

Adenovirus E1B19k binding protein B5 antibody; Adenovirus E1B19K-binding protein B5 antibody; BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody; BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody; BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody; BNI3L_HUMAN antibody; BNIP3a antibody; BNIP3H antibody; BNIP3L antibody; BNIP3L protein antibody; NIP3 like protein X antibody; NIP3-like protein X antibody; NIP3L antibody; NIX antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human BNIP3L protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12C4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BNIP3L, also known as NIX, is a BH3-only protein that plays a central role in programmed mitochondrial clearance through mitophagy. This protein is essential for the selective elimination of mitochondria during erythrocyte maturation and serves as a critical regulator of cell death pathways. BNIP3L has garnered significant research attention for its involvement in hypoxia-induced autophagy, cardiac homeostasis, and tumor suppression, making it a valuable target for studies spanning cancer biology, metabolic regulation, and developmental processes.

This recombinant monoclonal antibody, clone 12C4, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity against human BNIP3L, ensuring reproducible results across experiments and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The affinity-chromatography purification yields a highly pure reagent suitable for sensitive detection applications.

Validation studies demonstrate robust performance across multiple platforms. Immunohistochemistry staining in paraffin-embedded human kidney tissue and colorectal cancer specimens at 1:100 dilution reveals clear target detection using standard citrate buffer antigen retrieval protocols on automated Leica Bond systems. Flow cytometry analysis using the 786-O renal cell carcinoma line confirms intracellular detection capability, with distinct signal separation from isotype controls when cells are fixed and permeabilized.

Whether investigating mitophagy mechanisms, exploring BNIP3L's role in tumor microenvironment adaptation, or examining its function in tissue-specific contexts, this antibody provides the experimental flexibility researchers need. The validated performance in both tissue sections and cell-based assays supports diverse cell biology research applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

Gene References into Functions

The results demonstrate that Nix can serve as an alternative mediator of mitophagy to maintain mitochondrial turnover, identifying Nix as a promising target for neuroprotective treatment in PINK1/Parkin-related Parkinson's disease. PMID: 28281653
Here the authors present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. PMID: 28442745
p75(NTR) and NIX may play critical roles in intracerebral hemorrhage-induced neuronal apoptosis in vitro and in vivo. PMID: 27726026
Data suggest that targeting BNIP3L protein in wild-type p53 tumor suppressor colon cancer cells is an anticancer strategy activating iron depletion signaling and the mitophagy-related cell death pathway. PMID: 26517689
regulates mitophagy during hypoxia, whereas NIX is required for mitophagy during development of the erythroid lineage. PMID: 25753537
Nix protein positively regulates NF-kappaB activation in gliomas PMID: 22984526
mitochondrial ROS and NIX are essential factors for Mieap-induced accumulation of lysosome-like organelles within mitochondria PMID: 21264228
Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation PMID: 20010802
NIX binds to TSAP6 in tumor cells and has a role in apoptosis PMID: 12606722
The proapoptotic factor Nix is a highly regulated effector of growth during terminal erythroid maturation. PMID: 12663450
Bnip3L, capable of attenuating tumorigenicity, mediates p53-dependent apoptosis under hypoxia. PMID: 15607964
NIX is required for the selective elimination of mitochondria, however, because mitochondrial clearance, in the absence of NIX, is arrested at the stage of mitochondrial incorporation into autophagosomes and autophagosome maturation. PMID: 18048346
Homozygous deletions in a number of biologically important genes were found in prostate cancer cell lines, including PPP2R2A and BNIP3L identified in this study. PMID: 18670647

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Subcellular Location

Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane; Single-pass membrane protein. Note=Colocalizes with SPATA18 at the mitochondrion outer membrane.

Protein Families

NIP3 family

Database Links

HGNC: 1085

OMIM: 605368

KEGG: hsa:665

STRING: 9606.ENSP00000370003

UniGene: Hs.131226

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Mitophagy

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