Product Details

Uniprot No.

Target Names

CAMK1

Alternative Names

Calcium / calmodulin dependent protein kinase 1 antibody; Calcium / calmodulin dependent protein kinase I antibody; Calcium / calmodulin dependent protein kinase type 1 antibody; Calcium/calmodulin dependent protein kinase 1 antibody; Calcium/calmodulin dependent protein kinase I antibody; Calcium/calmodulin dependent protein kinase type 1 antibody; Calcium/calmodulin-dependent protein kinase type 1 antibody; CaM K1 antibody; CaM KI antibody; CaM kinase 1 alpha antibody; CAM kinase 1 antibody; CaM kinase I alpha antibody; CaM kinase I antibody; CaM-KI antibody; CaMK 1 antibody; CAMK I antibody; CaMK1 alpha antibody; CAMK1 antibody; CAMK1 PEN antibody; CaMKI alpha antibody; CaMKI-alpha antibody; KCC1A_HUMAN antibody; MGC120317 antibody; MGC120318 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human CAMK1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5G8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Calcium/calmodulin-dependent protein kinase type 1 plays a central role in calcium-mediated signal transduction, particularly within neuronal systems where it contributes to synaptic plasticity, learning, and memory formation. As an upstream activator in the CaMK cascade, CAMK1 phosphorylates downstream targets in response to elevated intracellular calcium levels, making it a key molecule for researchers investigating neurodevelopmental processes, cognitive function, and calcium signaling pathways.

This recombinant monoclonal antibody, clone 5G8, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring reproducible results across extended studies and multi-site collaborations. The rabbit host origin combined with monoclonal specificity provides excellent signal-to-noise characteristics for detecting endogenous CAMK1 protein.

Validation studies demonstrate robust performance in Western blot applications, where the antibody detects a band at the predicted molecular weight of 41 kDa. Testing across human cell lines including A549, HEK293, and MCF7, as well as mouse and rat brain tissue lysates, confirms reliable cross-species reactivity spanning human, mouse, and rat samples. This broad species compatibility supports comparative studies and translational research models. The recommended working dilution range of 1:500 to 1:5000 for Western blot provides flexibility to optimize signal intensity based on target abundance in your specific samples.

For neuroscience researchers exploring calcium signaling mechanisms, synaptic function, or kinase cascade regulation, this antibody delivers the specificity and reproducibility needed to generate publication-quality data with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation. Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.

Gene References into Functions

The mRNA expressions of PPP3CB and MEF2C were significantly up-regulated, and CAMK1 and PPP3R1 were significantly down-regulated in mitral regurgitation(MR) patients compared to normal subjects. Moreover, MR patients had significantly increased mRNA levels of PPP3CB, MEF2C and PLCE1 compared to aortic valve disease patients PMID: 27907007
Barettin has inhibitory activity against two protein kinases related to inflammation, namely the receptor-interacting serine/threonine kinase 2 (RIPK2) and calcium/calmodulin-dependent protein kinase 1alpha (CAMK1alpha). PMID: 26466644
CAMK1 functional regulation subnetwork in hepatocellular carcinoma PMID: 24825433
this study suggests that the tightly linked regulatory loop comprised of the SIK2-PP2A and CaMKI and PME-1 networks may function in fine-tuning cell proliferation and stress response. PMID: 24841198
It is one of Ca(2+)-sensing proteins and substantial age- and Alzheimer disease(AD)-related alterations in Ca(2+)-sensing proteins most likely contribute to selective vulnerability of basal forebrain cholinergic neurons to degeneration in AD. PMID: 24461366
Fbxl12-induced CaMKI degradation attenuates p27 phosphorylation at these sites in early G1 and iii) activation of CaMKI during G1 transition followed by p27 phosphorylation appears to be upstream to other p27 phosphorylation events PMID: 23707388
structures of three CaMKIalpha truncates in apo form and in complexes with ATP PMID: 23028635
CaMKI was significantly upregulated in adrenal adenomas. PMID: 21249615
variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, have roles in memory and general cognitive abilities PMID: 19844571
CaMKI is involved in angiotensin II and K(+) stimulation of CYP11B2 transcription and the capacity of the adrenal to produce aldosterone PMID: 12193581
we cloned a novel calmodulin-kinase (CaM-KIdelta) from HeLa cells and characterized its activation mechanism. CaM-KIdelta exhibits Ca(2+)/CaM-dependent activity that is enhanced in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)alpha PMID: 12935886
calcium/calmodulin-dependent protein kinase I regulates of cyclin D1/Cdk4 complexes PMID: 14754892
Calcium/calmodulin-dependent protein kinase I inhibits neuronal nitric-oxide synthase activity through serine 741 phosphorylation PMID: 15251453
Dioxin-mediated up-regulation of aryl hydrocarbon receptor target genes is dependent on the calcium/calmodulin/CaMKIalpha pathway. PMID: 18089838

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily

Tissue Specificity

Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.

Database Links

HGNC: 1459

OMIM: 604998

KEGG: hsa:8536

STRING: 9606.ENSP00000256460

UniGene: Hs.434875

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Code	CSB-RA074783A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:A549 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain lysate(30µg) All lanes: CaMKI antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time：120s
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CAMK1 Recombinant Monoclonal Antibody

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Target Background