CBX3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA935966A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), A431 whole cell lysate(30µg), A375 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), HT-29 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: CBX3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 24 kDa Observed band size: 21 kDa Exposure time：2min40s IHC image of CSB-RA935966A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA935966A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA935966A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q13185

Target Names

CBX3

Alternative Names

CBX 3 antibody; CBX3 antibody; CBX3_HUMAN antibody; Chromobox homolog 3 (HP1 gamma homolog; Drosophila) antibody; Chromobox homolog 3 antibody; Chromobox protein homolog 3 antibody; GAMMA antibody; HECH antibody; Heterochromatin like protein 1 antibody; Heterochromatin protein 1 homolog gamma antibody; Heterochromatin protein HP1 gamma antibody; HP1 gamma antibody; HP1 gamma homolog antibody; HP1Hs gamma antibody; Modifier 2 protein antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human CBX3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21H8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CBX3, also known as HP1 gamma, serves as a critical component of heterochromatin organization and gene silencing machinery. As a member of the heterochromatin protein 1 family, CBX3 recognizes and binds methylated histone H3 lysine 9, playing essential roles in chromatin remodeling, transcriptional regulation, and maintenance of genome stability. Its involvement in epigenetic mechanisms makes it a valuable target for researchers investigating nuclear signaling pathways, cell differentiation, and chromatin dynamics.

This recombinant monoclonal antibody, generated from a synthesized peptide derived from human CBX3, offers the reproducibility and consistency that demanding epigenetics research requires. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies, ensuring your experimental conditions remain constant across projects.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms reliable detection in diverse human cell lines including HeLa, A431, A375, Jurkat, SH-SY5Y, and HT-29, as well as mouse NIH/3T3 cells, supporting cross-species reactivity for comparative studies. The observed band at 21 kDa, slightly smaller than the predicted 24 kDa molecular weight, likely reflects post-translational processing or protein conformation effects common with chromatin-associated proteins. Immunohistochemistry validation in human placenta tissue demonstrates suitability for tissue-based investigations, while immunofluorescence staining in HepG2 cells reveals clear nuclear localization patterns consistent with CBX3's known biological function. Flow cytometry analysis in A431 cells further extends its utility for single-cell applications.

Whether you're exploring heterochromatin dynamics, investigating epigenetic regulation, or characterizing nuclear protein complexes, this antibody provides a dependable tool for advancing your chromatin biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).

Gene References into Functions

c-Myc was capable of upregulating HP1gamma by directly binding to the E-box element in the first intron of HP1gamma gene, and the upregulated HP1gamma, in turn, repressed the expression of miR-451a by enhancing H3K9 methylation at the promoter region of miR-451a. PMID: 28967902
CBX3 was overexpressed in pancreatic adenocarcinoma, which was associated with poor prognosis. CDK1 knockdown attenuated the cell cycle transition, proliferation and invasion of CBX3-overexpressing PAAD cells. PMID: 29903985
CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
HP1gamma is present in the nucleus and the cytoplasm interacting with actin, a protein complex that may exert different functions depending on its subcellular localization. PMID: 29208528
CBX3 has a role in promoting tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma PMID: 29462646
The deletion of CBX3 directly enforces the expression of CDK6 and p21. PMID: 28193906
These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin gene expression. PMID: 28154185
these data underscore the importance of IDRs 1, 2, and 3 in defining the structural and dynamic properties of HP1gamma, discoveries that have both mechanistic and potentially biomedical relevance. PMID: 26680990
In contrast to HP1alpha, HP1gamma could not recognize H3K9me3 in extended nucleosomes. PMID: 26319017
this study identified miR-30a as a tumor-suppressive microRNA that targets HP1gamma in vitro and in vivo to specifically suppress the growth of colorectal cancer in mouse xenograft models. PMID: 26333808
BARD1 interacts with H3K9me2 through HP1 and plays a critical role in retention of a BRCA1/BARD1 complex at the site of DNA damage. PMID: 25634209
CBX3 and CRABP2 expression was markedly increased in lung cancer tissues PMID: 24751108
our data suggested that HP1gamma plays an important role in promoting non-small cell lung cancer PMID: 24981246
compartmentalized and total HP1gamma protein expression is increased in PCa and that expression correlates with clinical outcomes better than Gleason score. PMID: 23522301
HP1gamma affects the spontaneous differentiation potential of P19 cells, and might play major roles in the decision of cell fates in pluripotent stem cells. PMID: 23313480
Identification of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma. PMID: 22870217
Loss of CBX3 results in global RNA processing defect. PMID: 22684280
The Cbx3 chromodomain binds with comparable affinities to all of the methylated H3K9, H1K26 and G9aK185 peptides. PMID: 22514736
HIV-1 Vpr displaces heterochromatin protein 1-alpha and heterochromatin protein 1-gamma from chromatin, resulting in premature chromatid separation. PMID: 21875947
we show distinct cell-type- and cancer-stage-associated patterns of key heterochromatin marks: heterochromatic adaptor proteins HP1alpha and HP1gamma, compared with the gammaH2AX marker of endogenously activated DNA damage response. PMID: 20695923
HP1gamma localizes to telomeres in S phase, where it is required to establish/maintain cohesion PMID: 21865325
this study reveals that human NIPBL is a novel protein recruited to DSB sites, and the recruitment is controlled by MDC1, RNF168 and HP1gamma. PMID: 21784059
The Ca2+ channel beta4c subunit interacts with heterochromatin protein 1 gama via a PXVXL binding motif. PMID: 21220418
HP1 gamma binds CD44 pre-mRNA inside the variant region of CD44. PMID: 21358630
Under oxidative conditions, HP1gamma dimerized and held TIF1beta in a chromatin component and inhibited its repression ability. PMID: 20675861
Pax3 represses transcription through a novel mechanism involving competition between corepressor KAP1 and the heterochromatin-binding protein HP1gamma PMID: 16945326
Suv39H1, HP1gamma and histone H3Lys9 trimethylation play a major role in chromatin-mediated repression of integrated HIV-1 gene expression. PMID: 17245432
In non-differentiated cells, transcriptional intermediary factor TIF1beta/heterochromatin protein HP1 interaction occurs only within euchromatin and involves selectively HP1beta and HP1gamma, but not HP1alpha. PMID: 17381543
Overexpression of HP1-GAMMA in wing imaginal discs caused abnormal wings. PMID: 17575412
present an investigation of DNA methylation and histone modification marks across the HNRPA2B1-CBX3 locus in primary peripheral blood mononuclear cells (PBMCs) to characterise the chromatin structure that underlies UCOE activity PMID: 18032920
Heterochromatin protein gamma coordinately regulates CCN2/CTGF transcription by interacting with nuclear MMP3. PMID: 18172013
the spatial proximities among a constellation of functionally related sites that are found within euchromatic regions of the cell nucleus including: HP1gamma, RNA polymerase II, matrin 3, and SAF-A sites PMID: 18618731

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Subcellular Location

Nucleus.

Database Links

HGNC: 1553

OMIM: 604477

KEGG: hsa:11335

STRING: 9606.ENSP00000336687

UniGene: Hs.381189

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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