Code | CSB-RA004847MA4HU |
Size | US$210 |
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CUSABIO meticulously generated the CCR8 recombinant monoclonal antibody through a systematic procedure. Initially, B cells were extracted from the immunized animal's spleen, employing the recombinant human CCR8 protein as the immunogen during the immunization process. Subsequently, RNA isolation from the B cells was followed by cDNA synthesis through reverse transcription. By utilizing the cDNA as a template, the gene encoding the CCR8 antibody was extended using a degenerate primer and inserted into a recombinant vector. The recombinant vector was then introduced into host cells via transfection, facilitating the expression of the CCR8 recombinant monoclonal antibodies. These antibodies were harvested from the cell culture supernatant and subjected to purification using affinity chromatography. Rigorous validation testing FC was conducted to verify this antibody's reactivity with human CCR8 protein, ensuring its specificity and reliability.
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