Code | CSB-RA004847MA3HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:50-1:200 |
The synthesis of the CCR8 recombinant monoclonal antibody is carried out with meticulous precision to ensure its exceptional quality and specificity. The process begins by isolating B cells from the spleen of an immunized animal, where the recombinant human CCR8 protein serves as the immunogen. RNA is extracted from the B cells and converted into cDNA through reverse transcription. Using specific primers designed for the antibody constant regions, the CCR8 antibody genes are amplified and inserted into an expression vector. The vector is then introduced into host cells through transfection, allowing for the production of the CCR8 recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for a wide range of applications. To ensure its reliability and effectiveness, the antibody undergoes FC analysis to validate its specificity and functionality in detecting human CCR8 protein.
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