Code | CSB-RA004847MA2HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:50-1:200 |
The creation of the CCR8 recombinant monoclonal antibody involves a meticulous process to ensure its exceptional quality and specificity. B cells are initially isolated from the spleen of an immunized animal, with the recombinant human CCR8 protein used as the immunogen. RNA is then extracted from the B cells and converted into cDNA through reverse transcription. The CCR8 antibody genes are amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells to facilitate the production of the CCR8 recombinant monoclonal antibody. Following a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. CUSABIO performs FC to validate the antibody's specificity and functionality in detecting human CCR8 protein. Through this rigorous production process, a reliable and effective CCR8 recombinant monoclonal antibody is generated, playing a critical role in diverse CCR8-related research.
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