CD53 Recombinant Monoclonal Antibody

CD53 is a member of the tetraspanin superfamily, a group of transmembrane proteins that organize membrane microdomains and regulate diverse cellular processes including adhesion, migration, and signal transduction. Expressed predominantly on leukocytes, CD53 plays important roles in immune cell function and has been implicated in lymphocyte activation, T cell co-stimulation, and inflammatory responses. Its involvement in immune regulation makes it a valuable target for researchers investigating hematological malignancies, autoimmune conditions, and the tumor microenvironment.

This recombinant monoclonal antibody, clone 7F6, offers the consistency and reliability that demanding research applications require. Produced using recombinant technology with a sequence-defined rabbit IgG framework, it eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The antibody was raised against a synthetic peptide derived from human CD53, ensuring precise epitope targeting.

Validation in immunohistochemistry demonstrates clear performance in paraffin-embedded human pancreatic cancer tissue, where the antibody was tested at 1:100 dilution using a standard citrate buffer antigen retrieval protocol. This validation in tumor tissue is particularly relevant for researchers examining CD53 expression patterns in cancer contexts or investigating immune cell infiltration within the tumor microenvironment. The recommended working dilution range of 1:50-1:200 for IHC provides flexibility for optimization across different tissue types and detection systems.

Whether you are characterizing leukocyte populations in tissue sections, exploring tetraspanin biology in cancer research, or investigating immune cell dynamics, this antibody provides a dependable tool for advancing your understanding of CD53 function in human samples.