Code | CSB-RA004995A0HU |
Size | US$210 |
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The CDC25B recombinant monoclonal antibody is produced using DNA recombinant technology and in vitro genetic manipulation. It involves immunizing animals with a synthesized peptide derived from human CDC25B to isolate B cells and select positive B cells. These positive B cells undergo screening and identification of individual clones. The light and heavy chains of the CDC25B antibody are then amplified using PCR and inserted into a plasmid vector. This recombinant vector is transfected into a host cell line for antibody expression. The CDC25B recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It specifically binds to human CDC25B protein and is recommended for ELISA.
CDC25B is a phosphatase enzyme that plays a critical role in the regulation of the cell cycle. Its main function is to activate the CDK complexes that drive the progression of the cell cycle through the G2/M checkpoint. This activation occurs by dephosphorylating specific residues on the CDK complexes, which allows them to bind to and phosphorylate their target substrates, leading to mitosis and cell division. CDC25B also plays a role in DNA damage response and repair. It can be activated in response to DNA damage, leading to increased cell cycle arrest and DNA repair mechanisms.
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