CDC25C Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA004996A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg) All lanes: CDC25C antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 60 kDa Exposure time: 120s IHC image of CSB-RA004996A0HU diluted at 1:80 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of Hela cell with CSB-RA004996A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with CSB-RA004996A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

P30307

Target Names

CDC25C

Alternative Names

CDC 25 antibody; Cdc 25C antibody; CDC25 antibody; CDC25C antibody; Cell division cycle 25 homolog C antibody; Cell division cycle 25C antibody; Cell division cycle 25C protein antibody; Dual specificity phosphatase Cdc25C antibody; M phase inducer phosphatase 3 antibody; M-phase inducer phosphatase 3 antibody; Mitosis inducer CDC25 antibody; MPIP3 antibody; MPIP3_HUMAN antibody; Phosphotyrosine phosphatase antibody; PPP1R60 antibody; protein phosphatase 1, regulatory subunit 60 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CDC25C

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CDC25C, also known as M-phase inducer phosphatase 3, serves as a critical regulator of cell cycle progression by dephosphorylating and activating cyclin-dependent kinase 1, thereby triggering entry into mitosis. This dual specificity phosphatase has become an important focus in cell biology research, particularly in studies examining cell cycle checkpoints, DNA damage responses, and the mechanisms underlying uncontrolled proliferation in cancer.

This recombinant monoclonal antibody, generated from clone 3E6 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers can expect reliable performance across experiments and between lots, eliminating a common source of variability in long-term studies.

Validation testing demonstrates this antibody's versatility across multiple applications. Western blot analysis in K562 whole cell lysate reveals a band at approximately 60 kDa, slightly higher than the predicted 54 kDa molecular weight. This size difference likely reflects post-translational modifications such as phosphorylation, which is particularly relevant given CDC25C's role as a phosphatase that is itself heavily regulated by phosphorylation during cell cycle progression. Immunohistochemistry staining has been validated in paraffin-embedded human testis tissue using citrate buffer antigen retrieval, while immunofluorescence studies confirm detection in both HeLa and U251 cell lines, demonstrating utility across different cellular contexts including cervical carcinoma and glioblastoma models.

With validated performance in Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and ELISA, this antibody provides flexibility for researchers investigating cell cycle regulation, checkpoint signaling, and related areas of cell biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Gene References into Functions

Cells lacking ARID1A show enhanced AURKA transcription, which leads to the persistent activation of CDC25C, a key protein for G2/M transition and mitotic entry. PMID: 30097580
FHL1 increase inhibitory CDC25 phosphorylation by forming a complex with CHK2 and CDC25, and sequester CDC25 in the cytoplasm by forming another complex with 14-3-3 and CDC25, resulting in increased radioresistance in cancer cells. PMID: 28094252
CDC25A plays a novel role in regulating the malignant behavior of glioma stem cells as a part of Linc00152/miR-103a-3p/FEZF1/CDC25A axis. PMID: 28651608
Overexpression of the CDK1 and CDC25A may have a role in the pathogenesis of the NFPA. PMID: 28004354
Mdm2 overexpression and Cdc25C downregulation delay cell cycle progression through the G2/M phase. PMID: 28806397
Xanthatin functions as a DNA-damaging agent in non-small cell lung carcinomas by activating Chk1-mediated DDR and lysosome-mediated degradation of Cdc25C. PMID: 29074359
Myelodysplastic syndrome -related P95 point mutants of SRSF2 lead to alternative splicing of CDC25C in a manner that is not dependent on the DNA damage response. PMID: 27552991
The aim of this review is to shed light on the role of four different phosphatases (PTEN, PP2A, CDC25 and DUSP1) in five different solid tumors (breast cancer, lung cancer, pancreatic cancer, prostate cancer and ovarian cancer), in order to better understand the most frequent and aggressive primary cancer of the central nervous system, glioblastoma. PMID: 28801478
Data show that TRIB2-mediated degradation of CDC25C is associated with lysine-48-linked CDC25C polyubiquitination driven by the TRIB2 kinase-like domain. PMID: 27563873
High expression of pCHK2-Thr68 was associated with decreased patient survival (p = 0.001), but was not an independent prognostic factor. Our results suggest that pCHK2-Thr68 and pCDC25C-Ser216 play important roles in breast cancer and may be potential treatment targets PMID: 27801830
the biology of the activation/deactivation of CDC25 by kinases/phosphatases to maintain the level of CDK-cyclin activities and thus the genomic stability PMID: 27038604
the knockdown of CDC25C can reduce both the radiotherapy sensitivity and the proliferation activity of EC9706 cells. PMID: 27188256
results identify CDC25C as a downstream target of the mutated tyrosine kinase FLT3-ITD affecting cell-cycle regulation in a model of AML PMID: 27919943
Suggest that the p53-p21-DREAM-CDE/CHR pathway regulates p53-dependent repression of Survivin, CDC25C, and PLK1 in HCT116 cells. PMID: 26595675
These miR-142-3p functioned as a tumor suppressor by targeting CDC25C. PMID: 26805039
Cdc25C negatively regulates proapoptotic ASK1 in a cell cycle-dependent manner and may play a role in G2/M checkpoint-mediated apoptosis. PMID: 25633196
Recurrent CDC25C mutations drive malignant transformation in familial platelet disorder to acute myelogenous leukaemia. PMID: 25159113
we conclude that inhibition of KIF22 suppresses cancer cell proliferation by delaying mitotic exit through the transcriptional upregulation of CDC25C. PMID: 24626146
These findings indicate that DHM inhibits the growth of hepatocellular carcinoma (HCC) cells via G2/M phase cell cycle arrest through Chk1/Chk2/Cdc25C pathway PMID: 24002546
Purification and biochemical analysis of catalytically active human cdc25C dual specificity phosphatase PMID: 23567337
protein plays a role in regulating PCa cell growth, and androgen treatments, but not EGF, greatly increase Cdc25C protein levels in AS PCa cells, which is in part by decreasing its degradation. PMID: 23637932
dose-dependent Cdc25c phosphatase acts as an early G2-phase checkpoint, thus indicating mechanistic importance in the low-dose hyper-radiosensitivity and induced radioresistance transition PMID: 22843362
data suggest that the maintenance of CDC25 activity does not fully rely on the thioredoxin reductase system in breast cancer cells, even in the presence of a major oxidative stress PMID: 22360685
cloning and functional analysis of Cdc25C PMID: 22394631
MMEQ induced G2/M arrest through the promotion of cdc25c in TSGH8301 cells. PMID: 22021033
Inhibition of CK2 activity by three different inhibitors led to a down-regulation of the level of cdc25C. PMID: 21750987
Two additional sites other than Ser216 in the widely studied cell division cycle (Cdc) protein 25C, whose function depends on 14-3-3 binding, were identified. PMID: 21189416
The results show for the first time that in human mitosis, distinct phospho-isoforms of cdc25C exist with different localizations and interacting partners. PMID: 20668692
ATM and Chk1/2 mediated phosphorylation of cdc25c plays a major role in cell cycle arrest induced by pectenotoxin2. PMID: 20514472
CDC25C and phospho-CDC25C (Ser216) play a crucial role in the pathogenesis and/or progression of vulvar squamous cell carcinomas. PMID: 20500813
Adventitious arsenate reductase activity of the catalytic domain of the human and Cdc25C phosphatases PMID: 20025242
Analysis of cell cycle profile and cell cycle regulatory proteins indicated that arsenite arrested cell cycle at G(2)/M phase, partially through induction of cell division cycle 25 (Cdc25) isoform C (Cdc25C) degradation via ubiquitin-proteasome pathways PMID: 11842186
results suggest that Plk1 phosphorylates Cdc25C on Ser198 and regulates nuclear translocation of Cdc25C during prophase PMID: 11897663
role of degradation by oxidative stress in induction of cell cycle arrest PMID: 11925443
Human CDC25B and CDC25C differ by their ability to restore a functional checkpoint response after gene replacement in fission yeast PMID: 12099692
Ca2+ promotes erythrocyte band 3 tyrosine phosphorylation via dissociation of phosphotyrosine phosphatase from band 3. PMID: 12175337
phosphorylation by Chk2 PMID: 12386164
CDC25C is phosphorylated on Ser 214 during mitosis which, in turn, prevents phosphorylation of Ser 216. HeLa cells depeleted of endogenous CDC25C, when treated with exogenous CDC25C, had a substantial delay to mitotic entry. PMID: 12766774
cdc25C not only plays a role at the G2/M transition but also in the modulation of DNA replication PMID: 12857880
CDC25C translocation to the cell nucleus upon entry into mitosis is coordinated by Plk3 PMID: 14968113
binding to VPR protein in human cell lines correlates with G2 arrest PMID: 14972559
downregulation of Cdc25C is mediated by p53 via two independent mechanisms, one involving direct binding to the cdc25C promoter PMID: 15574328
Vpr promotes cell cycle arrest at the G(2)/M phase by facilitating association of 14-3-3 and Cdc25C PMID: 15708996
Vitamin C transiently arrests cancer cell cycle progression in S phase and G2/M boundary by modulating the kinetics of activation of CDC25C. PMID: 15887239
Data suggests that CDC25C might play an important role in prostate cancer progression and could be used to monitor and predict the aggressiveness of this disease. PMID: 16000564
Data suggest that Pim-1 activates Cdc25C by a direct phosphorylation and can thereby assume the function of a positive cell cycle regulator at the G2/M transition. PMID: 16356754
Crystallization experiments of PLK1 protein in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C yield crystals suitable for X-ray diffraction analysis. PMID: 16582488
These results demonstrate that the MAPK ERK signaling pathway contributes to the p53-independent antiproliferative functions of p14ARF. Furthermore, they identify a new mechanism by which phosphorylation at serine 216 participates to Cdc25C inactivation. PMID: 16582626
Phosphorylation of cdc25c can be used to test whether a pharmacologic inhibitor of Plk1 would exert the same cellular effects as interference with Plk1 on an mRNA level. PMID: 16648550
Chk1-mediated phosphorylation of Cdc25C plays a major role in response to LOR-mediated G(2)/M arrest. Although the Chk1-mediated cell growth arrest in a tumor cell line. PMID: 16649252

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Subcellular Location

Nucleus.

Protein Families

MPI phosphatase family

Database Links

HGNC: 1727

OMIM: 157680

KEGG: hsa:995

STRING: 9606.ENSP00000321656

UniGene: Hs.656

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