CDC5L Recombinant Monoclonal Antibody

Code CSB-RA858712A0HU
Size US$210
Order now
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
    All lanes: CDC5L antibody at 1.3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 93 KDa
    Observed band size: 100 KDa
  • IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-RA858712A0HU diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of A549 cells with CSB-RA858712A0HU at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating CDC5L in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA858712A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA858712A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

Have Questions? Leave a Message or Start an on-line Chat

Product Details

Uniprot No.
Target Names
CDC5L
Alternative Names
CDC5 antibody; CDC5 cell division cycle 5-like (S. pombe) antibody; Cdc5 related protein antibody; CDC5-LIKE antibody; Cdc5-like protein antibody; Cdc5l antibody; CDC5L_HUMAN antibody; CEF1 antibody; Cell division cycle 5 S. pombe homolog of antibody; Cell division cycle 5-like protein antibody; dJ319D22.1 antibody; KIAA0432 antibody; PCDC5RP antibody; Pombe Cdc5 related protein antibody; Pombe Cdc5-related protein antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human CDC5L
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
1E3
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
IP 1:200-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The gene sequence of the CDC5L monoclonal antibody was obtained by CUSABIO from splenocytes isolated from animals with human CDC5L synthetic peptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the CDC5L recombinant monoclonal antibody. It's an affinity-chromatography purified rabbit IgG antibody. This CDC5L recombinant monoclonal antibody detects the CDC5L protein from human sources in ELISA, WB, IHC, IF, and IP applications.

CDC5L regulates the G2/M transition and is involved in the catalytic step of pre-messenger RNA (mRNA) splicing and DNA damage repair. It is also substantially expressed in some human somatic tumors, such as gliomas, osteosarcoma, and hepatocellular carcinoma.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Gene References into Functions
  1. Study demonstrates that the levels of Prp19 and Cdc5L are overexpressed in hepatocellular carcinoma (HCC). Prp19 binds with Cdc5L and its downregulation results in reduction of Cdc5L. Mechanistic insights reveal that silencing Prp19 compromises translation activity of Cdc5L and facilitates lysosome-induced degradation of Cdc5L in HCC cells. PMID: 28387715
  2. CDC5L might play an important role in glioma cell survival and disease progression. PMID: 26490980
  3. Cdc5L was highly expressed in hepatocellular carcinoma. Overexpression of Cdc5L favors cell cycle progress of HCC cells, while downregulation of Cdc5L results in cell cycle arrest at G2/M phase and reduced cell proliferation of HCC cells. PMID: 26553251
  4. The results show that CTNNBL1 enhances the association of CWC15 and CDC5L, both core Prp19 complex proteins and identify an overlap in the region of CDC5L that binds either CTNNBL1 or CWC15 suggesting the two proteins might exchange places in the complex. PMID: 26130721
  5. Cdc5L is a key regulator of mitotic progression. PMID: 24675469
  6. The N-terminal ARM-repeat domain of CTNNBL1 provides a binding site for CDC5L, a binding partner in the Prp19-CDC5L complex. PMID: 24598747
  7. CTNNBL1 is a novel nuclear localization sequence-binding protein that recognizes RNA-splicing factors CDC5L and Prp31 PMID: 21385873
  8. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
  9. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. PMID: 20467437
  10. Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites PMID: 19641227
  11. CDC5 may function in pre-mRNA processing and cell cycle progression PMID: 14515018
  12. the interaction between CDC5L and PLRG1 is essential for pre-mRNA splicing PMID: 14576297
  13. Results indicate that CDC5L is the most likely candidate oncogene for the 6p12-p21 amplicon found in osteosarcoma. PMID: 18567798
  14. Phosphorylation of CDC5L at threonines 411 and 438 within recognition sequences for CDKs are required for CDC5L-mediated pre-mRNA splicing. PMID: 18583928

Show More

Hide All

Involvement in disease
A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.
Subcellular Location
Nucleus. Nucleus speckle. Cytoplasm.
Protein Families
CEF1 family
Tissue Specificity
Ubiquitously expressed in both fetal and adult tissues.
Database Links

HGNC: 1743

OMIM: 602868

KEGG: hsa:988

STRING: 9606.ENSP00000360532

UniGene: Hs.485471

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*