CDCA5 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA436367A0HU
Size	US$210
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Image	Western Blot Positive WB detected in:Raji whole cell lysate(30µg) All lanes: Sororin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 28 kDa Observed band size: 35 kDa Exposure time：3min IHC image of CSB-RA436367A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cell with CSB-RA436367A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA436367A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q96FF9

Target Names

CDCA5

Alternative Names

Sororin (Cell division cycle-associated protein 5) (p35), CDCA5

Species Reactivity

Human

Immunogen

A synthesized peptide from human CDCA5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CDCA5, also known as Sororin, plays an essential role in sister chromatid cohesion during cell division. This protein stabilizes the cohesin complex by antagonizing WAPL-mediated cohesin release, ensuring proper chromosome segregation during mitosis. Given its critical function in cell cycle regulation, CDCA5 has emerged as a significant target in cancer research, where its overexpression has been linked to tumor progression and poor prognosis across multiple malignancies.

This recombinant monoclonal antibody, generated against a synthesized peptide from human CDCA5, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced from a single rabbit clone (20F9), it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The affinity-purified IgG format ensures minimal background and reliable performance across your applications.

Validation data demonstrates robust performance across multiple platforms. Western blot analysis in Raji whole cell lysate reveals a band at approximately 35 kDa, larger than the predicted 28 kDa molecular weight—a difference likely attributable to post-translational modifications such as phosphorylation, which is known to regulate CDCA5 function during the cell cycle. Immunohistochemistry staining in human lung cancer tissue sections shows clear target detection, while immunofluorescence in A549 cells and flow cytometry analysis in HeLa cells confirm utility for both imaging and quantitative single-cell applications.

Whether investigating mitotic regulation, exploring CDCA5 as a potential biomarker, or studying cohesin dynamics, this antibody provides the versatility and reliability needed for meaningful results across cell biology and oncology research programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.

Gene References into Functions

High CDCA5 expression is associated with hepatocellular carcinoma. PMID: 30015982
These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion. PMID: 29383807
Silencing of CDCA5 suppresses proliferation of gastric cancer cells by inducing G1-phase arrest via downregulating CCNE1. PMID: 29326043
Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2-PP2A. PMID: 26951638
The results suggest that CDCA5 functions as a critical gene supporting oral squamous cell carcinoma progression and that targeting CDCA5 may be a useful therapeutic strategy for oral squamous cell carcinoma. PMID: 26497678
phosphorylation plays unexpected roles in regulating the subcellular localization of Sororin. PMID: 26177583
the C-terminus of Sororin functions as an anchor binding to SA2, which facilitates other conserved motifs on Sororin to interact with other proteins to regulate sister chromatid cohesion and separation. PMID: 25608232
We propose that the Prp19 complex and the splicing machinery contribute to the establishment of cohesion by promoting Sororin accumulation during S phase, and are, therefore, essential to the maintenance of genome stability. PMID: 25092791
A transcriptome-wide analysis revealed that SNW1 or PRPF8 depletion affects the splicing of specific introns in a subset of pre-mRNAs, including pre-mRNAs encoding the cohesion protein sororin and the APC/C subunit APC2. PMID: 25257309
Targeting p35/Cdk5 signalling via CIP-peptide promotes angiogenesis in hypoxia. PMID: 24098701
Data show that the MEK-ERK pathway regulates cell cycle-related neuronal apoptosis (CRNA) by elevating the levels of cyclin D1, and the increase in cyclin D1 attenuates the activation of cyclin-dependent kinase 5 (cdk5) by its neuronal activator p35. PMID: 22833568
Sororin is phosphorylated by Cdk1/cyclin B at prophase and acts as a docking protein to bring Plk1 into proximity with SA2, resulting in the phosphorylation of SA2 and the removal of cohesin complexes from chromosomal arms. PMID: 21987589
the interaction of the highly conserved motif at the C terminus of sororin with the cohesin complex is critical to its ability to mediate sister chromatid cohesion. PMID: 21115494
Transactivation of CDCA5 and its phosphorylation at Ser209 by ERK play an important role in lung cancer proliferation. PMID: 20551060
data indicate that sororin interacts with chromatin-bound cohesin and functions during the establishment or maintenance of cohesion in S or G2 phase, respectively PMID: 17349791
Sororin protects centromeric cohesion in response to the spindle checkpoint, but prevents the removal of cohesion by a mechanism independent of the anaphase-promoting complex. PMID: 17361102

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Subcellular Location

Nucleus. Chromosome. Cytoplasm. Note=Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.

Protein Families

Sororin family

Database Links

HGNC: 14626

OMIM: 609374

KEGG: hsa:113130

STRING: 9606.ENSP00000275517

UniGene: Hs.434886

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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