CRCP Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA134191A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), Mouse kidney tissue lysate(30µg) All lanes: CRCP polymerase beta antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 22 kDa Exposure time：3min Immunofluorescence staining of MCF-7 cell with CSB-RA134191A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA134191A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O75575

Target Names

CRCP

Alternative Names

DNA-directed RNA polymerase III subunit RPC9 (RNA polymerase III subunit C9) (Calcitonin gene-related peptide-receptor component protein) (CGRP-RCP) (CGRP-receptor component protein) (CGRPRCP) (HsC17), CRCP

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human CRCP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CRCP, also known as CGRP-receptor component protein, serves a dual role in cellular biology as both a critical component of the calcitonin gene-related peptide receptor complex and as RNA polymerase III subunit C9. This bifunctional protein participates in CGRP-mediated signaling pathways involved in vasodilation, pain transmission, and neurogenic inflammation, while simultaneously contributing to the transcription of small non-coding RNAs essential for cellular function. These intersecting roles make CRCP a compelling target for researchers investigating neuropeptide signaling, vascular biology, and transcriptional regulation.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human CRCP, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures that every lot performs identically, eliminating variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis successfully detects CRCP in both Jurkat whole cell lysates and mouse kidney tissue, with the observed band at 22 kDa appearing slightly larger than the predicted 17 kDa molecular weight—a shift commonly attributable to post-translational modifications such as glycosylation. Immunofluorescence staining in MCF-7 cells reveals clear subcellular localization patterns, while flow cytometry analysis in SH-SY5Y neuroblastoma cells confirms robust detection in suspension-based applications. This cross-species reactivity with human and mouse samples provides flexibility for translational research designs.

Whether investigating CGRP receptor biology in migraine research, exploring RNA polymerase III function, or examining CRCP expression patterns across tissue types, this antibody delivers the performance consistency essential for publication-quality results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.; Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.

Gene References into Functions

data reinforce and clarify our understanding of CGRP receptor localization, and provide evidence that antagonist antibodies bind to target sites in the trigeminovascular system of primates that are involved in the pathology of migraine PMID: 27155150
CGRP family of receptors displays both ligand- and RAMP-dependent signaling bias among the Galphas, Galphai, and Galphaq/11 pathways. PMID: 27566546
In this review, the influence of the CGRP family peptides in reproduction and pregnancy is explored and discussed. PMID: 25015815
This study demonstrates the detailed expression of CGRP and its receptor components within spinal trigeminal nucleus in the brainstem and in the spinal cord at C1-level PMID: 22074408
The extracellular protein domains corresponding to residues 23-133 of calcitonin receptor-like receptor and residues 26-117 of receptor activity-modifying protein 1 (RAMP1) are shown to be sufficient for formation of a stable, folded monodisperse complex. PMID: 20099900
Expression of CGRP-RCP was detected in CD34+ cells and also in some mature granulocyte samples, providing evidence that CGRP-RCP may be regulated independently of RAMP1 and CRLR in hematopoietic cells. PMID: 11937264
human CGRP-RCP is the genuine orthologue of C17. CGRP-RCP was found to functionally replace C17 in Deltarpc17 yeast cells; CGRP-RCP was identified in a purified human Pol III preparation. PMID: 12482973
inhibition of ACE seems to augment CGRP catabolism. PMID: 16406141
Increased mRNA expression CGRP-RCP in gravid myometrium supports the possibility of involvement of CGRP in the control of myometrial contractility. PMID: 17853344
A peptide mimetic demonstrates that 12 residues of family B member CGRP receptor proximal C-terminus are required for this receptor's expression and localization at the cell membrane. PMID: 18636754
analysis of the effects of antagonists to the CGRP receptor PMID: 19053766

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Subcellular Location

Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Eukaryotic RPC9 RNA polymerase subunit family

Tissue Specificity

Ubiquitous. Most prevalent in testis.

Database Links

HGNC: 17888

OMIM: 606121

KEGG: hsa:27297

STRING: 9606.ENSP00000378736

UniGene: Hs.300684

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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