Code | CSB-RA914307A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:50-1:200 |
The production of the CSTB recombinant monoclonal antibody involves the following steps: Immunization and B cell isolation: An animal was immunized with a synthesized peptide derived from human CSTB, and B cells were subsequently isolated from its spleen. RNA extraction and cDNA synthesis: RNA was extracted from the isolated B cells, and reverse transcription was performed to convert the RNA into cDNA. Amplification and vector construction: The gene encoding the CSTB antibody was amplified using a degenerate primer through PCR, and the amplified gene was inserted into a vector. Transfection and antibody expression: The recombinant vector carrying the CSTB antibody gene was transfected into host cells, allowing for the expression of the CSTB recombinant monoclonal antibodies. Antibody harvesting and purification: The CSTB recombinant monoclonal antibodies were harvested from the cell culture supernatant and purified using affinity chromatography, ensuring their high quality and specificity. Antibody validation: The CSTB recombinant monoclonal antibody underwent rigorous validation, including extensive ELISA and IF testings, to confirm its specific reactivity with human CSTB protein.
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