CYP4A11 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA843868A0HU
Size	US$210
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Image	Western Blot Positive WB detected in:Mouse Kdiney whole cell lysate(30µg), Rat Kdiney whole cell lysate(30µg) All lanes: Lipoma preferred partner antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 59/50 kDa Observed band size: 50 kDa Exposure time：180s IHC image of CSB-RA843868A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA843868A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing HepG2 cells stained with CSB-RA843868A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q02928

Target Names

CYP4A11

Alternative Names

Cytochrome P450 4A11 (20-hydroxyeicosatetraenoic acid synthase) (20-HETE synthase) (CYP4AII) (CYPIVA11) (Cytochrome P-450HK-omega) (Cytochrome P450HL-omega) (Fatty acid omega-hydroxylase) (Lauric acid omega-hydroxylase) (Long-chain fatty acid omega-monooxygenase) (EC 1.14.13.205), CYP4A11, CYP4A2

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from Human CYP4A11 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19A7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid. Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver. Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure. Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency. May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening. Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer. Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids. Has little activity toward prostaglandins A1 and E1.

Gene References into Functions

Haplotype G-C-A of CYP4A11 was associated with increased risk of coronary artery disease. PMID: 29484037
Gene-gene interaction between rs1126742 and rs3890011 and gene-environment interaction between rs1126742 and smoking were associated with increased EH risk. PMID: 28534704
Heme-thiolate sulfenylation of human cytochrome P450 4A11 functions as a redox switch for catalytic inhibition. PMID: 28533430
These results point to a potential 20-HETE dependence of intrarenal angiotensinogen production and ANGII receptor type 1 activation that are associated with increases in NCC and SGK1 and identify elevated P450 4A11 activity and 20-HETE as potential risk factors for salt-sensitive human hypertension by perturbation of the renal renin-angiotensin axis. PMID: 27298316
CYP4A11 Variants are associated with Ischemic Stroke. PMID: 27087514
The results suggest that individuals carrying the alleles, K276T and S353G, might exhibit higher catalysis of CYP4A11 PMID: 27793475
CYP4A1l rs9333025 GG and CYP4F2 rs2108622 GG two-loci interaction significantly increases the risk for IS and an elevated 20-HETE level. PMID: 26959478
Single nucleotide polymorphism of CYP4A11 gene is associated with Plaque in Patients with Ischemic Stroke. PMID: 26423716
The present study focused on 10 CYP4A11 variant alleles and evaluated their functional characteristics using arachidonic acid as the substrate in a COS-7 cell-based expression system. PMID: 25760539
To evaluate the associations between four single-nucleotide polymorphisms (SNPs) in CYP4A11 and CYP4F2 and ischemic stroke (IS) PMID: 25734770
The CC genotype and C allele of the CYP4A11 gene were associated with essential hypertension in the male western Chinese Han population. PMID: 24164311
individuals homozygous for the CYP4A11 rs3890011 C allele, blood pressure is resistant to mineralocorticoid receptor antagonism, but sensitive to ENaC inhibition, consistent with ENaC activation. PMID: 25064769
Results indicate that both the transfer of an electron to the ferrous.O2 complex and C-H bond-breaking limit the rate of CYP4A11 ( cytochrome P450 4A11) omega-oxidation. PMID: 25203493
This meta-analysis suggests the CYP4A11 T8590C polymorphism may be a risk factor for hypertension. PMID: 24931260
suggests there is a significant association between the CYP4A11 T8590C variant and essential hypertension, especially in Caucasians. The case-control study did not find a significant association among the Han Chinese population PMID: 24278241
The results suggest that the CYP4A11 GG genotype was a high risk factor for hypertension. PMID: 24535879
This meta-analysis revealed that the RGS2 1891-1892del TC polymorphism and CYP4A11 T8590C polymorphism were associated with hypertension risk. PMID: 23859711
The CYP4A11 8590C allele was also associated with low HDL-C in women. PMID: 21912424
rs3890011 maybe a novel polymorphism of the CYP4A11 gene associated with CAD in a Han Chinese population. PMID: 22327816
The GG genotype of rs3890011 and the G-G-T haplotype in the CYP4A11 gene could be a useful genetic marker of CAD in Han populations in China. PMID: 23085321
Single-nucleotide polymorphisms of the human CYP4A11 gene appear to have no association with myocardial infarction in Japanese. PMID: 22804341
We confirmed that the CYP4A11 (8590T>C) functional polymorphism exhibits inter-ethnic frequency differences and an association with hypertension. PMID: 21617944
The loss-of-function CYP4A11 8590C allele is associated with a diagnosis of hypertension and, in normotensive individuals, with higher blood pressure regardless of salt intake. PMID: 21873888
Data suggest that results could be helpful for further investigations of the potential role of CYP4A variants in the genetic susceptibility to cardiovascular diseases such as arterial hypertension. PMID: 21820496
In normotensive individuals, the CYP4A11 rs4660980 polymophism was associated with both systolic and diastolic blood pressure in men. A common variant on CYP4A11 was associated with blood pressure in a Chinese population. PMID: 21326303
Association of common variants of CYP4A11 with stroke in Han Chinese population is reported. PMID: 20130494
In patients with established and stable coronary artery disease the 434SS variant of CYP4A11 F434 is associated with pronounced coronary vasoconstriction. PMID: 19615687
CYP4A11 expression can be induced by glucocorticoids and peroxisome proliferators PMID: 12464261
gene and protein analysis of CYP4A11 show that it is expressed in kidney PMID: 12464262
A variant of the human CYP4A11 (T8590C) encodes for a monooxygenase with reduced 20-HETE synthase activity. The association of the T8590C variant with hypertension supports its role as a polygenic determinant of blood pressure control in humans. PMID: 15611369
Our data strengthen the association between the T8590C polymorphism of CYP4A11 and hypertension and suggest a recessive mode of inheritance. PMID: 16144986
Genetic (T8590C) and environmental (insulin) factors impair 20-HETE responses to salt in human hypertension. The T8590C polymorphism of CYP4A11 encodes an enzyme with reduced catalytic activity. PMID: 18227405
Essential hypertension is associated with the TC + TT genotype of rs1126742 in the human CYP4A11 gene PMID: 18300855
The CYP4A11 8590CC genotype is associated with increased blood pressure in black men with hypertensive nephrosclerosis and is associated with adverse clinical outcomes in those with baseline proteinuria. PMID: 18385420
In humans that polymorphisms of the CYP4F2 and CYP4A11 genes have opposite effects on 20-hydroxyeicosatetraenoic acid excretion. PMID: 18391101
a functional variant (-845A/G) of CYP4A11 is significantly associated with hypertension and appears to be a novel candidate for a predisposing factor for hypertension PMID: 18936345
PPARalpha contributes to the maintenance of basal CYP4A11 expression and mediates CYP4A11 induction in response to fibrates or fasting; increased exposure to growth hormone down-regulates CYP4A11 expression in liver PMID: 19366684

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Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Expressed in liver. Expressed in S2 and S3 segments of proximal tubules in cortex and outer medulla of kidney.

Database Links

HGNC: 2642

OMIM: 601310

KEGG: hsa:1579

STRING: 9606.ENSP00000311095

UniGene: Hs.1645

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Vascular smooth muscle contraction

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