Code | CSB-RA871395A0HU |
Size | US$210 |
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The production of the DAXX antibody by CUSABIO involves several steps. Initially, an animal is immunized with a synthetic peptide derived from human DAXX. The resulting DAXX antibody is then sequenced, and the antibody gene is synthesized. CUSABIO further clones the DAXX antibody-coding genes into plasma vectors, which are subsequently transfected into mammalian cells using a lipid-based transfection reagent. After transient expression, the recombinant antibodies specific to DAXX are harvested and characterized. CUSABIO purifies the DAXX recombinant monoclonal antibody through affinity chromatography from the culture medium. This purified antibody is suitable for detecting human DAXX protein in ELISA.
DAXX is a multifunctional, widely expressed protein that plays a role in transcriptional control, apoptosis, carcinogenesis, and antiviral defense, among other cellular functions. DAXX has different biological functions in the modulation of either pro-apoptosis or anti-apoptosis depending on the cell type and signaling pathway it regulates. It serves as an anti-apoptotic factor by inhibiting the p53 function by stabilizing MDM2 in many cancer cells. DAXX overexpression is frequently seen in various cancers and is associated with tumorigenesis, disease progression, and treatment resistance.
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