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DLL3 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA882142A1HU
Size US$210
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Image
  • Untransfected HEK293 cells surface (green line) and transfected Human DLL3 HEK293 stable cells surface (red line) were stained with anti-DLL3 antibody (rovalpituzumab-like) (2µl/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG1 Fc antibody and analyzed with flow cytometry.
  • The Binding Activity of DLL3 with Anti-DLL3 Recombinant Antibody.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 (CSB-MP3536MOV) at 2 μg/mL can bind Anti-DLL3 Recombinant Antibody(CSB-RA882142A1HU), the EC50 is 1.625-2.702 ng/mL.
  • The Binding Activity of DLL3 with Anti-DLL3 Recombinant Antibody.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 (CSB-MP882142HU) at 2 μg/mL can bind Anti-DLL3 Recombinant Antibody(CSB-RA882142A1HU), the EC50 is 1.102-1.707 ng/mL.
  • The purity of DLL3 was greater than 95% as determined by SEC-HPLC
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Product Details

Uniprot No.
Q9NYJ7
Target Names
DLL3
Alternative Names
Delta-like protein 3 (Drosophila Delta homolog 3) (Delta3), DLL3
Species Reactivity
Human
Immunogen
Recombinant Human DLL3 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
7B7
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols
ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The development of the DLL3 recombinant monoclonal antibody involves a meticulous step-by-step process to ensure its exceptional quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, with the recombinant human DLL3 protein used as the immunogen. The RNA extracted from the B cells is converted into cDNA through reverse transcription. The DLL3 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, enabling the production of the DLL3 recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. Rigorous characterization assays, including ELISA and FC analysis, are performed to validate the antibody's specificity and functionality in detecting human DLL3 protein.

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Target Background

Function
Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.
Gene References into Functions
  1. these results reveal that DLL3 is expressed in tumor specimens from most patients with small cell lung cancer PMID: 29290251
  2. Results indicated that DLL3 expression was silenced in hepatocellular carcinoma (HCC) cells by DNA methylation and was more readily affected by histone acetylation than histone methylation (H3K9me2 or H3K27me3). PMID: 29512761
  3. our results indicated epidermal growth factor-like domain multiple 7 protein participates in growth hormone-secreting pituitary adenoma proliferation and invasion regulation via Notch2/DLL3 signaling pathway. These findings raised the possibility that epidermal growth factor-like domain multiple 7 protein might serve as a useful biomarker to assess growth hormone-secreting pituitary adenoma invasion and prognosis PMID: 28705113
  4. The Dll3 was rarely detectable in the para-carcinoma tissues, but positive in 82.1% of non-small cell cancer tissues. PMID: 28007595
  5. Both global haplotype and individual haplotype analyses showed that the haplotypes of SNP1/SNP2/SNP3/SNP4/SNP5 did not correlate with the disease (P >0.05). Together, these data suggest that genetic variants of the DLL3 gene are not associated with CS in the Chinese Han population. PMID: 27472720
  6. DLL3 was silenced by methylation in human human hepatocellular carcinoma and it negatively regulates the growth of human hepatocellular carcinoma cells. PMID: 23337976
  7. We suggest that the three human DLL3 mutations associated with spondylocostal dysplasia are also functionally equivalent to the Dll3(neo) null allele in mice. PMID: 11923214
  8. mutations in DLL3 cause a consistent pattern of abnormal vertebral segmentation in spondylocostal dysostosis PMID: 12746394
  9. no novel or previously described mutations are present in our cohort, indicating that DLL3 mutations may not be a major cause of congenital scoliosis. PMID: 15717203
  10. The intracellular region of Notch ligands Dll1 and Dll3 regulates their trafficking and signaling activity PMID: 18676613

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Involvement in disease
Spondylocostal dysostosis 1, autosomal recessive (SCDO1)
Subcellular Location
Membrane; Single-pass type I membrane protein.
Database Links

HGNC: 2909

OMIM: 277300

KEGG: hsa:10683

STRING: 9606.ENSP00000205143

UniGene: Hs.127792

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Related pathways
Notch signaling pathway Th1 and Th2 cell differentiation Target Therapy
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