Product Details

Uniprot No.

Target Names

Alternative Names

Dual specificity phosphatase 6 antibody; Dual specificity phosphatase 6 isoform a antibody; Dual specificity protein phosphatase 6 antibody; Dual specificity protein phosphatase PYST1 antibody; DUS6_HUMAN antibody; DUSP 6 antibody; DUSP 6a antibody; Dusp6 antibody; DUSP6a antibody; HH19 antibody; MAP kinase phosphatase 3 antibody; Mitogen activated protein kinase phosphatase 3 antibody; Mitogen-activated protein kinase phosphatase 3 antibody; MKP 3 antibody; MKP-3 antibody; MKP3 antibody; PYST 1 antibody; PYST1 antibody; Serine/threonine specific protein phosphatase antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human DUSP6

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DUSP6, also known as MKP-3 or PYST1, serves as a critical negative regulator of the ERK signaling pathway, functioning as a dual specificity phosphatase that dephosphorylates both threonine and tyrosine residues on activated ERK1/2. This regulatory role positions DUSP6 at the center of research into cell proliferation, differentiation, and oncogenic signaling, making it a compelling target for investigators studying MAPK pathway dynamics and feedback mechanisms in both normal physiology and disease states.

This recombinant monoclonal antibody, generated in rabbit host against a synthetic peptide derived from human DUSP6, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments conducted months or years apart.

Validation testing demonstrates reliable performance across multiple platforms. Western blot analysis of HepG2 whole cell lysate detects a clean band at the predicted 42 kDa molecular weight, confirming specificity for the target protein at dilutions ranging from 1:500 to 1:2000. Flow cytometry applications have been validated using HeLa cells with intracellular staining protocols, showing clear separation from isotype control at dilutions between 1:50 and 1:200. This application flexibility allows researchers to move seamlessly between protein expression quantification and single-cell analysis within the same experimental framework.

Whether investigating ERK pathway feedback loops, characterizing phosphatase expression in cancer models, or exploring developmental signaling networks, this antibody provides a dependable tool for human DUSP6 detection in your research program.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.

Gene References into Functions

Individuals are carrying DUSP6 rs2279574 AA and AC genotypes associated with an increased risk in developing lung squamous carcinoma in Han Chinese and with advanced NSCLC stages. PMID: 29578153
DUSP6 expression in skeletal muscle was reduced by 43% just after exercise and remained below pre-exercise level after 2 h recovery. PMID: 28989118
Data show that oxidative stress and MAP kinase phosphatase 3 (MKP3) inhibition play a critical role in procyanidin B2 3,3''-di-O-gallate (B2G2)-induced cell death in prostate cancer (PCa) cells through sustained activation of both ERK1/2 and AMPKalpha. PMID: 28876465
High DUSP6 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525
Authors demonstrate the broad applicability of this recombination-based method and they proved its potential to identify new drug targets via the identification of the tumor suppressor DUSP6 as potential synthetic lethal target in melanoma cell lines with BRAF V600E mutations and high DUSP6 expression. PMID: 28423600
PICSAR has a role in promoting cSCC progression via activation of extracellular signal-regulated kinase 1/2 signaling pathway by downregulating DUSP6 expression PMID: 27049681
DUSP6 rs2279574 SNPs was not associated with chemoradiotherapy response, whereas tumor regression, weight loss, clinical stage, and cigarette smoking were independent prognostic predictors for these Chinese patients with non-small cell lung cancer. PMID: 28231233
DNA samples from each patient were genotyped for DUSP6 and TOP2A SNPs PMID: 27156317
Suppression of the FOXA1/DUSP6 signaling pathway may contribute to the development of Hirschsprung disease. PMID: 26097584
These observations led us to conclude that increased TSH signaling overcomes OIS and is essential for B-RafV600E-induced papillary thyroid carcinogenesis. PMID: 25499223
Dusp6 expression was higher in progestin-sensitive atypical endometrial hyperplasia groups compared with progestin-resistant groups. After treatment, Dusp6 expression was upregulated in progestin-sensitive groups, but not in progestin-resistant groups. PMID: 25451692
Our results suggest that DUSP6 gene expression in tumour samples may be a prognostic marker in NSCLC PMID: 25344212
DUSP6 downregulated the expression of matrix metallopeptidase 3. PMID: 25241655
These results therefore indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype through dephosphorylating ERK1/2 PMID: 25414256
Downregulation of Dusp6, Sprouty4, and Sef--negative modulators of FGF2/ERK1/2 signaling--was present in eutopic endometria of adenomyosis, which may play critical roles in the development of adenomyosis. PMID: 24681741
Higher expression of DUSP6 in tumor tissue, than in peritumor tissue, is associated with the recurrence after curative resection of HCC, and the relative tumor DUSP6 expression has good power to predict the recurrence of HCC. PMID: 24709168
suggest an important role for DeltaNp63alpha in preventing cancer metastasis by inhibition of Erk2 signaling via MKP3 subscribing PMID: 23246965
Dusp6 overexpression is involved in the pathogenesis and development of human endometrial adenocarcinoma. PMID: 23419500
Increased DUSP6 expression is associated with papillary thyroid carcinoma. PMID: 24222120
Depletion of DUSP6 reduced the viability of cancer cell lines and increased the cytotoxicity of EGFR and other targeted inhibitors, and cytotoxic agents. PMID: 23839489
missense mutation, c.545C>T (p.Ser182Phe), in the DUSP6 gene may be genetically linked to the Class III malocclusion PMID: 23965468
DUSP6 is overexpressed in papillary and poorly differentiated thyroid carcinoma and contributes to neoplastic properties of thyroid cancer cells. PMID: 23132790
The results provide insights on the modulatory role of p53 in the survival pathway by up-regulating DUSP6. PMID: 23108049
study reports that naive CD4 T cells from elderly individuals have reduced signaling capacity of the ERK pathway; the underlying mechanism is an age-related decline in miR-181a expression and associated rise in levels of DUSP6 expression PMID: 23023500
ischemia/reperfusion inhibited eNOS expression by inactivation of ERK1/2, leading to decreased NO formation through a MKP-3-dependent mechanism in endothelial cells PMID: 22848708
DUSP6 plays an important role in the pathogenesis of bipolar disorder, particularly in women. PMID: 22155192
MESP2, HES7 and DUSP6 genes may not be involved in the etiopathogenesis of sporadic and non-syndromic CS in Chinese Han population. PMID: 22744456
A link between DUSP6 expression and high-risk features of papillary thyroid carcinoma suggested that DUSP6 is an important independent factor affecting the signaling pathways in established papillary thyroid carcinoma. PMID: 22535643
DUSP6 is important in melanoma and it plays a different role in the distinct subtype of mouse melanoma compared with that in classic human melanoma. PMID: 22171919
caspase-3 cleavage of MKP3 down-regulates MKP3 full length and renders active MKP3 fragments, which may participate in novel regulatory pathways controlling the subcellular localization and activation of ERK1/2 during apoptosis. PMID: 22504224
Studies identified a single gene DUSP6, whose expression was associated with sensitivity to GSK1120212. PMID: 22169769
Studies indicate that ERKs stimulate their own dephosphorylation by directly binding to phosphatases, such as MKP-3, and activating them. PMID: 22028468
Loss of DUSP6 is associated with esophageal squamous cell carcinoma and nasopharyngeal carcinoma. PMID: 21387288
DUSP6 plays an important role in cancer resistance in different subtypes of non-small cell lung carcinoma. PMID: 21680106
upregulation of DUSP6 exerts a tumor-promoting role in human glioblastomas exacerbating the malignant phenotype. PMID: 21499306
MKP3 not only controls the activities of ERK2 and p38alpha but also mediates cross-talk between these two MAPK pathways. PMID: 21454500
This study shows that post-transcriptional regulation is a key process in the control of DUSP6 expression. PMID: 20665674
DUSP6 methylation is a rare event in endometrial cancer. Silencing of the DUSP6 phosphatase is unlikely to contribute to constitutive activation of the ERK kinase cascade in endometrial cancer. PMID: 20638106
Dual specificity phosphatase 6 (DUSP6) is an ETS-regulated negative feedback mediator of oncogenic ERK signaling in lung cancer cells. PMID: 20097731
Angiotensin II-induced upregulation of MAP kinase phosphatase-3 mRNA levels mediates endothelial cell apoptosis PMID: 11998972
Results show that DUSP6 exerts apparent tumor-suppressive effects in vitro and suggest that DUSP6 is a strong candidate tumor suppressor gene at 12q22 locus. PMID: 12759238
Constitutive induction of p-Erk1/2 accompanied by reduced activities of protein phosphatases 1 and 2A and MKP3 due to reactive oxygen species during cellular senescence. PMID: 12840032
Study gives first direct evidence that the N-terminal domain of MKP3 mediates intramolecular dephosphorylation between the monomeric form of phosphorylated extracellular signal-regulated kinase and a monomer of MKP3. PMID: 14690430
cytoplasmic localization of MKP3 is mediated by a chromosome region maintenance-1 (CRM1)-dependent nuclear export pathway; the ability of MKP3 to cause the cytoplasmic retention of ERK2 requires both a functional kinase interaction motif and NES PMID: 15269220
Hypermethylation with modification of histone deacetylation play an important role in transcriptional suppression of DUSP6 in human pancreatic cancer PMID: 15824892
Results suggest that the abrogation of DUSP6 is associated exclusively with progression from pancreatic intraepithelial neoplasia to the invasive ductal carcinoma. PMID: 15832194
Results demonstrate that in the Korean population DUSP6 may contribute to the etiology of bipolar disorder, but not schizophrenia. PMID: 16491131
Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
even upon over-expression DUSP6 fails to inactivate ERK5, confirming that it is indeed an ERK1/2-specific DUSP PMID: 18280112
MKP3 can act to enhance DAT function and is a phosphatase involved in regulating dynamin-dependent endocysis PMID: 18434601

Hide All

Involvement in disease

Hypogonadotropic hypogonadism 19 with or without anosmia (HH19)

Subcellular Location

Cytoplasm.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily

Tissue Specificity

Expressed in keratinocytes (at protein level).

Database Links

HGNC: 3072

OMIM: 602748

KEGG: hsa:1848

STRING: 9606.ENSP00000279488

UniGene: Hs.298654

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Code	CSB-RA274139A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg) All lanes: DUSP6 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2min Overlay Peak curve showing Hela cells stained with CSB-RA274139A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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DUSP6 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background