EEF1G Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA149810A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SH-SY5Y whole cell lysate(20µg),Hela whole cell lysate(20µg), A549 whole cell lysate(20µg), COLO-205 whole cell lysate(20µg),HT-29 whole cell lysate(20µg),PC-3 whole cell lysate(20µg) All lanes: eEF1B gamma antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time：20s Immunofluorescence staining of Hela cell with CSB-RA149810A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA149810A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P26641

Target Names

EEF1G

Alternative Names

2610301D06Rik antibody; AA407312 antibody; eEF 1B gamma antibody; EEF 1G antibody; eEF-1B gamma antibody; EEF1G antibody; EF 1 gamma antibody; EF 1G antibody; EF-1-gamma antibody; EF1 gamma antibody; EF1G antibody; EF1G_HUMAN antibody; Elongation factor 1 gamma antibody; Elongation factor 1-gamma antibody; Eukaryotic translation elongation factor 1 gamma antibody; GIG 35 antibody; GIG35 antibody; MGC103354 antibody; MGC114210 antibody; MGC144723 antibody; MGC144724 antibody; MGC94929 antibody; Pancreatic tumor related protein antibody; PRO1608 antibody; Translation elongation factor eEF 1 gamma chain antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human EEF1G

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probably plays a role in anchoring the complex to other cellular components.

Gene References into Functions

Results show that eEF1Bgamma binds not only Che-1 and p53 transcripts but also their promoters. Its depletion significantly decreases p53 protein accumulation and slightly impacts on Che-1 accumulation in the mitochondria. These data support the notion that eEF1Bgamma, besides its canonical role in translation, is an RNA-binding protein and a key player in cellular stress responses. PMID: 27639846
eEF1gamma, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and shuttling/nursing the Vimentin mRNA PMID: 21217813

Tissue Specificity

Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.

Database Links

HGNC: 3213

OMIM: 130593

KEGG: hsa:1937

STRING: 9606.ENSP00000331901

UniGene: Hs.144835

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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