Code | CSB-RA585624A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
Geminin, encoded by the GMNN gene, is present during the S, G2, and M phases of the cell cycle and is degraded during the metaphase-anaphase transition by the anaphase-promoting complex (APC), which recognizes the destruction box sequence near the 50 ends of the geminin protein. Because reducing Geminin levels reduces Cdt1 accumulation and affects DNA replication, Geminin is implicated in licensing replication by boosting Cdt1 accumulation in mitosis. Geminin is a master regulator of cell-cycle progression that guarantees that DNA replication begins on time and that it does not repeat itself.
The first step in the preparation of recombinant GMNN antibody is to obtain the GMNN antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant GMNN antibody was obtained using Affinity-chromatography. This recombinant GMNN antibody has been validated for the detection of GMNN protein from Human in the ELISA, IF.There are currently no reviews for this product.