GNB2 Recombinant Monoclonal Antibody

GNB2, or guanine nucleotide-binding protein subunit beta-2, serves as a critical component of heterotrimeric G protein complexes that transduce signals from G protein-coupled receptors to downstream effectors. This beta subunit participates in diverse cellular processes including signal transduction cascades, receptor trafficking, and ion channel regulation, making it a valuable target for researchers investigating GPCR signaling pathways, neurobiology, and cellular communication mechanisms.

This recombinant rabbit monoclonal antibody (clone 10D6) offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human GNB2, the recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. Affinity chromatography purification further enhances signal clarity by removing non-specific immunoglobulins.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis at 1:1000 dilution reveals a clean band at approximately 35 kDa—slightly below the predicted 37 kDa molecular weight, a difference commonly attributable to post-translational processing or anomalous gel migration. Detection has been confirmed across an extensive panel of human cell lines including HeLa, U-251MG, SH-SY5Y, HEK293, HT-29, PC-3, and MCF-7, as well as mouse NIH/3T3 cells and mouse brain tissue, demonstrating reliable cross-species reactivity between human and mouse samples. Immunohistochemistry applications have been validated in paraffin-embedded human prostate cancer and kidney tissues at 1:100 dilution using citrate buffer antigen retrieval, showing clear cytoplasmic staining patterns consistent with GNB2's known subcellular localization.

This antibody supports investigations into G protein signaling, cancer biology, and neurological research where consistent, reproducible detection of GNB2 is essential.