HDAC6 Antibody

Datasheet
Code CSB-RA010242A0HU
Size US$350
Uniprot No. Q9UBN7
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
    All lanes: HDAC6 antibody at 1.1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 132, 115 KDa
    Observed band size: 160 KDa

  • IHC image of CSB-RA010242A0HU diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating HDAC6 in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA010242A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA010242A0HU (3μg) + HepG2 whole cell lysate (500μg)
    Lane 3: HepG2 whole cell lysate (20μg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Immunogen A synthesized peptide derived from human HDAC6
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Relevance Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Histone deacetylase 6, HD6, HDAC6, KIAA0901, JM21
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Gene Names HDAC6
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
Involvement in disease Chondrodysplasia with platyspondyly, distinctive brachydactyly, hydrocephaly, and microphthalmia (CDP-PBHM)
Subcellular Location Nucleus, Cytoplasm, Perikaryon, Cell projection, dendrite, Cell projection, axon
Protein Families Histone deacetylase family, HD type 2 subfamily
Database Links

HGNC: 14064

OMIM: 300272

KEGG: hsa:10013

STRING: 9606.ENSP00000334061

UniGene: Hs.6764

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