Code | CSB-RA167089A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
FC | 1:50-1:200 |
The HMGCL recombinant monoclonal antibody was meticulously developed by CUSABIO through a well-defined process. Initially, B cells were isolated from the spleen of an immunized animal, with the synthesized peptide derived from human HMGCL used as the immunogen. Following that, RNA was extracted from the B cells and converted into cDNA through reverse transcription. Using the cDNA as a template, the gene encoding the HMGCL antibody was amplified with a degenerate primer and cloned into a vector. The recombinant vector was then introduced into host cells via transfection, enabling efficient expression of the HMGCL recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and purified using affinity chromatography. The antibody has been tested for its specificity and reliability for the human HMGCL protein in four applications, including ELISA, WB, IHC, and FC.
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