HMGCS2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA693900A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate All lanes: HMGCS2 antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 51 kDa Observed band size: 55 kDa IHC image of CSB-RA693900A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA693900A0HU diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

P54868

Target Names

HMGCS2

Alternative Names

Hydroxymethylglutaryl-CoA synthase, mitochondrial (HMG-CoA synthase) (EC 2.3.3.10) (3-hydroxy-3-methylglutaryl coenzyme A synthase), HMGCS2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HMGCS2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the HMGCS2 recombinant monoclonal antibody involves a carefully orchestrated process that includes the following steps. Initially, B cells are isolated from an animal immunized with a synthesized peptide derived from human HMGCS2. Total RNA is extracted from the harvested B cells, and cDNA is synthesized through reverse transcription. The HMGCS2 antibody genes are then amplified using PCR with specific primers and cloned into an expression vector. This vector is transfected into host cells to facilitate the production of the HMGCS2 recombinant monoclonal antibody. Afterward, the antibody is purified from the cell culture supernatant using affinity chromatography. To ensure its quality, the purified antibody undergoes rigorous characterization and validation, including ELISA, WB, and IHC analysis, to confirm its specificity and ability to recognize human HMGCS2 protein. This systematic approach guarantees the generation of a reliable HMGCS2 recombinant monoclonal antibody suitable for various applications in the study of HMGCS2 protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.

Gene References into Functions

several nutrients as well as specific nutritional related hormonal conditions are able to affect significantly HMGCS2 gene expression. PMID: 28888048
miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma PMID: 28867541
this studies demonstrate that HMGCS2 functions as a transcriptional factor that binds to peroxisome proliferator-activated receptor alpha (PPARalpha), resulting in Src expression and activation in a metabolically independent manner PMID: 27816970
HMGCS2 shares with ketone bodies common features in autophagic clearance of APP, suggesting that ketone bodies play an important role in HMGCS2 regulation of the autophagy. PMID: 28320515
High expression of either HSD17B2 or HMGCS2 predicted poor susceptibility of rectal cancer to preoperative chemoradiotherapy. PMID: 25929810
FABP7 and HMGCS2 may have roles in apocrine differentiation categorizing apocrine carcinoma of the breast PMID: 25389781
In Fe-S cluster-deficient muscles, results show a dramatic up-regulation of the ketogenic enzyme HMGCS2 and the secreted protein FGF21 (fibroblast growth factor 21). PMID: 23943793
Human HMGCS2 regulates mitochondrial fatty acid oxidation and FGF21 expression PMID: 21502324
Ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2. PMID: 16940161

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Involvement in disease

3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D)

Subcellular Location

Mitochondrion.

Protein Families

HMG-CoA synthase family

Tissue Specificity

Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta

Database Links

HGNC: 5008

OMIM: 600234

KEGG: hsa:3158

STRING: 9606.ENSP00000358414

UniGene: Hs.59889

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PPAR signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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