HMGCS2 Antibody

Datasheet

Code	CSB-PA010567LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Rat kidney tissue All lanes: HMGCS2 antibody at 4.5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 53, 51 kDa Observed band size: 57 kDa IHC image of CSB-PA010567LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA010567LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA010567LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HMGCS2 Polyclonal antibody

Uniprot No.

P54868

Target Names

HMGCS2

Alternative Names

3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody; 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody; 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody; 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody; HMCS2_HUMAN antibody; HMG CoA synthase antibody; HMG-CoA synthase antibody; HMGCS 2 antibody; HMGCS2 antibody; Hydroxymethylglutaryl CoA synthase antibody; Hydroxymethylglutaryl CoA synthase mitochondrial antibody; Hydroxymethylglutaryl-CoA synthase antibody; mitochondrial antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Hydroxymethylglutaryl-CoA synthase, mitochondrial protein (426-508AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HMGCS2 Antibody (Product code: CSB-PA010567LA01HU) is Non-conjugated. For HMGCS2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010567LB01HU	HMGCS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA010567LC01HU	HMGCS2 Antibody, FITC conjugated
Biotin	CSB-PA010567LD01HU	HMGCS2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate.

Gene References into Functions

several nutrients as well as specific nutritional related hormonal conditions are able to affect significantly HMGCS2 gene expression. PMID: 28888048
miR-107-mediated decrease of HMGCS2 indicates poor outcomes and promotes cell migration in hepatocellular carcinoma PMID: 28867541
this studies demonstrate that HMGCS2 functions as a transcriptional factor that binds to peroxisome proliferator-activated receptor alpha (PPARalpha), resulting in Src expression and activation in a metabolically independent manner PMID: 27816970
HMGCS2 shares with ketone bodies common features in autophagic clearance of APP, suggesting that ketone bodies play an important role in HMGCS2 regulation of the autophagy. PMID: 28320515
High expression of either HSD17B2 or HMGCS2 predicted poor susceptibility of rectal cancer to preoperative chemoradiotherapy. PMID: 25929810
FABP7 and HMGCS2 may have roles in apocrine differentiation categorizing apocrine carcinoma of the breast PMID: 25389781
In Fe-S cluster-deficient muscles, results show a dramatic up-regulation of the ketogenic enzyme HMGCS2 and the secreted protein FGF21 (fibroblast growth factor 21). PMID: 23943793
Human HMGCS2 regulates mitochondrial fatty acid oxidation and FGF21 expression PMID: 21502324
Ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2. PMID: 16940161

Hide All

Involvement in disease

3-hydroxy-3-methylglutaryl-CoA synthase-2 deficiency (HMGCS2D)

Subcellular Location

Mitochondrion.

Protein Families

HMG-CoA synthase family

Tissue Specificity

Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta

Database Links

HGNC: 5008

OMIM: 600234

KEGG: hsa:3158

STRING: 9606.ENSP00000358414

UniGene: Hs.59889

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PPAR signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address