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HRAS/KRAS/NRAS Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA060625A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: SW620 whole cell lysate(20µg), HT29 whole cell lysate(20µg), Jurkat whole cell lysate(20µg), Hela whole cell lysate(20µg), HEK293 whole cell lysate(20µg), NIH/3T3 whole cell lysate(20µg)
    All lanes: Ras antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Exposure time：1min30s
  • Overlay Peak curve showing HepG2 cells stained with CSB-RA060625A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P01111 P01112 P01116
Target Names
HRAS/KRAS/NRAS
Alternative Names
NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human HRAS/KRAS/NRAS
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
6B2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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HRAS/KRAS/NRAS Antibodies

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