Code | CSB-RA783626A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:50-1:200 |
In the production of the HRH3 recombinant monoclonal antibody, in vitro expression systems are utilized, entailing the cloning of HRH3 antibody DNA sequences from immunoreactive rabbits. The immunogen used in this process is a synthesized peptide derived from the human HRH3 protein. Subsequently, the genes encoding the HRH3 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are transfected into host cells to enable antibody expression. Following expression, the HRH3 recombinant monoclonal antibody is purified through affinity chromatography and subjected to extensive testing in ELISA and FC applications. These tests affirm the antibody's reactivity with the human HRH3 protein.
HRH3 protein, as a histamine receptor, plays a central role in modulating neurotransmitter release in the central nervous system. Its functions extend to regulating histamine levels, influencing cognitive processes, sleep-wake cycles, appetite, and various physiological and neuropsychiatric processes.
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