Product Details

Uniprot No.

Target Names

Alternative Names

Heat shock 27kDa protein antibody; 28 kDa heat shock protein antibody; CMT2F antibody; DKFZp586P1322 antibody; epididymis secretory protein Li 102 antibody; Estrogen regulated 24 kDa protein antibody; Estrogen-regulated 24 kDa protein antibody; Heat shock 25kDa protein 1 antibody; Heat shock 27 kDa protein antibody; Heat shock 27kD protein 1 antibody; Heat shock 27kDa protein 1 antibody; Heat shock 28kDa protein 1 antibody; Heat Shock Protein 27 antibody; Heat shock protein beta 1 antibody; Heat shock protein beta-1 antibody; heat shock protein family B (small) member 1 antibody; HEL-S-102 antibody; HMN2B antibody; HS.76067 antibody; Hsp 25 antibody; HSP 27 antibody; Hsp 28 antibody; Hsp B1 antibody; Hsp25 antibody; HSP27 antibody; Hsp28 antibody; HspB1 antibody; HSPB1_HUMAN antibody; SRP27 antibody; Stress responsive protein 27 antibody; Stress-responsive protein 27 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HSPB1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HSPB1, commonly known as HSP27, is a small heat shock protein that plays a central role in cellular stress responses, protein folding, and apoptosis regulation. As a molecular chaperone, HSPB1 helps protect cells from thermal stress, oxidative damage, and other environmental insults, making it a critical target for researchers investigating signal transduction pathways, cancer biology, and cellular stress mechanisms.

This recombinant monoclonal antibody, clone 10C11, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The affinity-purified IgG format ensures high specificity for your target.

Extensive validation demonstrates this antibody's versatility across multiple applications. Western blot analysis confirms robust detection in a broad panel of human cell lines including HeLa, PC3, 293T, LO2, HT-29, and K562, with the observed band appearing at approximately 27 kDa rather than the predicted 23 kDa, a difference attributable to post-translational modifications such as phosphorylation that are characteristic of this stress-responsive protein. Immunohistochemistry validation in paraffin-embedded human pancreatic tissue and cervical cancer specimens confirms suitability for tissue-based studies. Additional validated applications include immunofluorescence staining in MCF-7 cells, immunoprecipitation from HeLa lysates, and flow cytometry analysis, providing flexibility for diverse experimental approaches.

Whether you are examining stress response pathways, investigating HSPB1's role in disease progression, or studying its chaperone functions, this antibody delivers the performance and consistency needed for reliable, publication-quality results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA010833A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, PC3 whole cell lysate, 293T whole cell lysate, LO2 whole cell lysate, HT-29 whole cell lysate, K562 whole cell lysate All lanes: Hsp27 antibody at 0.62μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 KDa Observed band size: 27 KDa IHC image of CSB-RA010833A0HU diluted at 1:61.9 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA010833A0HU diluted at 1:61.9 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-RA010833A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Immunoprecipitating Hsp27 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA010833A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA010833A0HU (3μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg) Overlay histogram showing Hela cells stained with CSB-RA010833A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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HSPB1 Recombinant Monoclonal Antibody

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