ICAM3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA097685A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg) All lanes: ICAM 3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 60 kDa Observed band size: 130 kDa Exposure time：1min IHC image of CSB-RA097685A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA097685A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

P32942

Target Names

ICAM3

Alternative Names

CD50 antibody; CDW 50 antibody; CDw50 antibody; ICAM 3 antibody; ICAM-3 antibody; ICAM-R antibody; ICAM3 antibody; ICAM3_HUMAN antibody; ICAMR antibody; Intercellular adhesion molecule 3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ICAM3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5D1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ICAM3, also known as CD50, plays a critical role in leukocyte adhesion and immune cell communication. As a member of the intercellular adhesion molecule family, ICAM3 facilitates the initial contact between T cells and antigen-presenting cells, making it an important target for researchers investigating immune signaling, inflammatory responses, and lymphocyte activation pathways.

This recombinant monoclonal antibody, clone 5D1, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents, ensuring reproducible results across extended studies. The rabbit IgG format, purified by affinity chromatography, delivers high specificity for human ICAM3.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis of Jurkat whole cell lysate, the antibody detects ICAM3 at an observed molecular weight of approximately 130 kDa, which is notably higher than the predicted 60 kDa. This size difference reflects the extensive glycosylation characteristic of ICAM3, a transmembrane glycoprotein known to carry multiple N-linked glycan modifications that significantly increase its apparent molecular weight. Immunohistochemistry validation confirms clear staining in paraffin-embedded human lymph node tissue and human gastric cancer tissue, with optimal results achieved at 1:100 dilution using citrate buffer antigen retrieval.

The flexibility to work across ELISA, western blot, and immunohistochemistry applications makes this antibody a versatile tool for signal transduction research. Whether examining ICAM3 expression in immune cell populations or investigating its role in pathological contexts, this antibody provides the performance characteristics needed for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.

Gene References into Functions

exploration of the underlying mechanism demonstrated that ICAM3 not only binds to LFA-1 with its extracellular domain and structure protein ERM but also to lamellipodia with its intracellular domain which causes a tension that pulls cells apart (metastasis). PMID: 29729315
Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
ICAM3 acts as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils. PMID: 23775590
this data clearly indicate that ICAM-3 promotes drug resistance via inhibition of apoptosis. PMID: 24177012
Intercellular adhesion molecule (ICAM)-3 mRNA is upregulated in non-adherent endothelial forming cells. PMID: 23144795
Results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX3. PMID: 22479382
analysis of activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and beta2 integrins PMID: 22396536
ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. PMID: 22117198
the molecular basis of allergen-induced Th2 cell polarization PMID: 22205703
the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively. PMID: 21712539
Single nucleotide polymorphisms in ICAM3 gene is associated with lymphoma. PMID: 21239057
induction of morphological polarization in primary T lymphocytes and Jurkat cells enhances Kidins220/ARMS colocalization with ICAM-3 PMID: 21381019
CCR1 antagonist, BX471, did not significantly alter ICAM-3 expression in relapsing-remitting multiple sclerosis patients. PMID: 20086017
ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB PMID: 19956847
no significant risk association was found for SARS infection for the ICAM-3 Asp143Gly SNP. PMID: 19801714
A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting PMID: 11784723
identification of DC-SIGN binding sites PMID: 11799126
interactions with DC-SIGN does not promote DC-SIGN mediated HIV-1 transmission PMID: 12021323
Expression of DC-SIGN and its ligand, ICAM-3, is found in substantial amounts only in RA synovium, suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP-1. PMID: 12571844
ICAM-3 is highly expressed on the surface of human eosinophils and has a role in the downregulation of GM-CSF production. PMID: 12743567
Relationship of intercellular adhesion molecule-3 and hepatocyte growth factor with amyloidosis A in chronic renal-failure patients. There was a higher density of intercellular adhesion molecule-3-positive cells in the patients with amyloidosis A. PMID: 14704632
ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via reactive oxygen species-dependent signaling. PMID: 14726630
acts as a costimulating molecule to increase HIV-1 transcription and viral replication, a process allowing productive infection of quiescent CD4+ T lymphocytes. PMID: 15163761
Expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. PMID: 15880373
The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of dendritic cells. PMID: 17145745
Patients with SARS homozygous for ICAM3 Gly143 showed significant association with higher lactate dehydrogenase levels and lower total white blood cell counts. PMID: 17570115
This is the first case of CD20 positive mycosis fungoides involving a lymph node to be reported in the literature. PMID: 18261116
extended alpha(L)beta(2) with an open headpiece is required for ICAM-3 adhesion PMID: 18354203

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, ICAM family

Tissue Specificity

Leukocytes.

Database Links

HGNC: 5346

OMIM: 146631

KEGG: hsa:3385

STRING: 9606.ENSP00000160262

UniGene: Hs.654563

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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