Code | CSB-RA575353A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
FC | 1:20-1:200 |
CUSABIO prepared the LDLR recombinant monoclonal antibody using protein and DNA recombinant technology. Initially, a synthesized peptide derived from human LDLR was used to immunize mice. After that, spleen cells were extracted from the immunized mice under aseptic conditions, and the total RNA was isolated from these cells. The cDNA synthesized from RNA reverse transcription was used as a template for PCR amplification of the LDLR antibody gene. The obtained gene was then introduced into a vector, which was subsequently transfected into host cells for culture. The LDLR recombinant monoclonal antibody was purified from the supernatant of the cell culture using affinity chromatography. This antibody underwent rigorous verification and can be used for detecting human LDLR protein in ELISA, IHC, IF, and FC experiments.
The LDLR protein is a transmembrane receptor that plays a crucial role in cholesterol homeostasis by regulating the uptake of LDL (low-density lipoprotein) particles from the blood into cells. When LDL particles bind to the extracellular domain of LDLR, the receptor undergoes a conformational change that triggers its internalization by endocytosis. The LDL particles are then delivered to lysosomes, where they are hydrolyzed, and the cholesterol is released into the cytoplasm. The freed cholesterol can either be utilized by the cell or can be re-esterified for storage. Mutations in the LDLR gene can lead to familial hypercholesterolemia.
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