MAGOH Recombinant Monoclonal Antibody

MAGOH is a core component of the exon junction complex, a multiprotein assembly deposited on mRNAs during splicing that plays essential roles in mRNA surveillance, nonsense-mediated decay, and translational regulation. As a highly conserved protein involved in post-transcriptional gene regulation, MAGOH has become an important target for researchers investigating RNA processing mechanisms, splicing dynamics, and their connections to cellular proliferation and disease states.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human MAGOH, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports reliable data across extended studies and collaborative projects. The rabbit IgG format and affinity-chromatography purification ensure high specificity for your target.

Validation studies demonstrate robust performance in both immunofluorescence and flow cytometry applications. Immunofluorescence staining of A-431 cells at dilutions ranging from 1:50 to 1:200 reveals clear nuclear localization consistent with MAGOH's established role in mRNA processing. Flow cytometry analysis using Jurkat cells shows distinct positive signal separation from isotype control, confirming reliable detection in suspension cell preparations. These validated human cell lines provide researchers with established starting points for optimizing detection in their own experimental systems.

Whether you are investigating exon junction complex assembly, exploring nonsense-mediated decay pathways, or examining the broader landscape of epigenetic and nuclear signaling events, this antibody provides a dependable tool for advancing your understanding of post-transcriptional regulation in human cells.