MAP3K2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA444035A0HU
Size	US$210
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Image	Immunofluorescence staining of Hela cell with CSB-RA444035A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA444035A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9Y2U5

Target Names

MAP3K2

Alternative Names

M3K2_HUMAN antibody; Map3k2 antibody; MAPK/ERK kinase kinase 2 antibody; MEK kinase 2 antibody; MEKK 2 antibody; MEKK2b antibody; Mitogen activated protein kinase kinase kinase 2 antibody; Mitogen-activated protein kinase kinase kinase 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MAP3K2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1A4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MAP3K2, also known as MEKK2, functions as a critical upstream kinase in the MAPK signaling cascade, where it phosphorylates and activates MEK kinases to regulate cellular responses including proliferation, differentiation, and stress adaptation. This positioning within the signaling hierarchy makes MAP3K2 a valuable target for researchers investigating signal transduction mechanisms, inflammatory pathways, and oncogenic processes where aberrant MAPK signaling contributes to disease progression.

This recombinant monoclonal antibody, clone 1A4, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers can expect reliable performance across experiments and between lot numbers, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The rabbit host and monoclonal nature provide high specificity for human MAP3K2, with affinity chromatography purification ensuring minimal background interference.

Validation studies demonstrate effective performance in both immunofluorescence and flow cytometry applications using HeLa cells. Immunofluorescence staining at dilutions between 1:50 and 1:200 reveals clear cytoplasmic localization patterns when counter-stained with DAPI, while flow cytometry analysis shows distinct positive population shifts compared to isotype controls, confirming specific target recognition in fixed and permeabilized cells. These validated single-cell analysis capabilities make this antibody well-suited for studies examining MAP3K2 expression patterns, subcellular distribution, and population-level expression heterogeneity.

For researchers exploring MAPK pathway regulation, inflammatory signaling networks, or kinase-dependent cellular responses, this antibody provides a dependable tool for characterizing MAP3K2 in human cell models across multiple experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7. Plays a role in caveolae kiss-and-run dynamics.

Gene References into Functions

Compound 1s displayed activity in cell-based assays in which it inhibited ERK5 pathway activation in cells and inhibited cell migration in a scratch assay. Thus, we have identified a scaffold that has promising potential to be developed into a highly selective and potent inhibitor of MEKK2. Information from these SAR studies provides specific guidance for the future design of MEKK2 inhibitor probes. PMID: 29309787
miR-34c-3p may regulate triple-negative breast cancer progression by directly targeting the 3'-untranslated region of mitogen-activated protein kinase kinase kinase 2 (MAP3K2). PMID: 28069384
Study found miR-186 expression significantly decreased in lung cancer tissues and cells and MAP3K2 expression increased in the same cancer tissues. Also, results confirmed that MAP3K2 is a target gene of miR-186 and both expression correlated with prognosis. PMID: 27498924
SMYD3-mediated methylation of MAP3K2 increases mutant K-Ras-induced activation of ERK1/2. (Review) PMID: 25382779
Restoration of miR17/20a in solid tumor cells enhances the natural killer cell antitumor activity by targeting Mekk2 PMID: 24801835
HBXIP activated ERK1/2 through up-regulating MEKK2. PMID: 25304384
MEKK2 has a novel function as a regulator of ubiquitylation-dependent paxillin redistribution in breast tumour cells. PMID: 25190348
EBV microRNA BART 18-5p targets MAP3K2 to facilitate persistence in vivo by inhibiting viral replication in B cells. PMID: 25012295
Inhibitors of apoptosis proteins regulate myogenic differentiation by directly suppressing MEKK2/3-MEK5-ERK5 signaling. PMID: 24975362
methylation of MAP3K2 by SMYD3 increases MAP kinase signalling and promotes the formation of Ras-driven carcinomas PMID: 24847881
results strongly support a role for MEKK2 as a regulator of signaling that modulates breast tumor cell spread area and migration through control of focal adhesion stability PMID: 24491810
Data suggest that MEKK2 and MLK3 represent untargeted kinases in tumor biology for potential therapeutic development. PMID: 22139075
Hepatitis B virus X protein enhances the growth of hepatoma cells through upregulation of MEKK2. PMID: 21804577
Calcium regulation of EGF-induced ERK5 activation is mediated through interaction of MEKK2 with the adaptor protein Lad1. PMID: 20830310
MEKK2 is a potent activator of the c-Jun N-terminal kinase pathway in fibroblast-like synoviocytes in rheumatoid arthritis. PMID: 14734742
Unlike ERK5 and MEK5, their upstream activator MEKK2 is localized mainly in the cytosol of resting cells, and translocates into the nucleus upon EGF stimulation PMID: 15075238
MEKK2 is dimerized and activated by the novel protein Mip1. PMID: 15988011
Through interaction with MEKK2, XIAP functions in an ubiquitin ligase dependent manner to evoke a second wave of NF-kappaB activation, resulting in the modulation of NF-kappaB target gene expression. PMID: 18761086
Loss of MADD expression resulted in reduced Grb2 and Sos1/2 recruitment to the TNFR1 complex and decreased Ras and MEKK1/2 activation PMID: 19289468
The ability of MEKK2 to discriminate tumor from normal cells was orthogonally confirmed. PMID: 19690195

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Subcellular Location

Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily

Database Links

HGNC: 6854

OMIM: 609487

KEGG: hsa:10746

STRING: 9606.ENSP00000343463

UniGene: Hs.145605

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

MAPK signaling pathway Gap junction

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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