MARK3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA091918A0HU
Size	US$210
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Image	IHC image of CSB-RA091918A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA091918A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA091918A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P27448

Target Names

MARK3

Alternative Names

C-TAK1 antibody; Cdc25C associated protein kinase 1 antibody; Cdc25C-associated protein kinase 1 antibody; cTAK1 antibody; ELKL motif kinase 2 antibody; EMK-2 antibody; Emk2 antibody; ETK 1 antibody; KIAA4230 antibody; KP78 antibody; MAP microtubule affinity regulating kinase 3 antibody; MAP/microtubule affinity-regulating kinase 3 antibody; Mark3 antibody; MARK3_HUMAN antibody; Par 1a antibody; PAR1A antibody; Protein kinase STK10 antibody; Ser/Thr protein kinase PAR-1 antibody; Serine threonine protein kinase p78 antibody; Serine/threonine-protein kinase p78 antibody; SerThr protein kinase PAR 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human MARK3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MARK3, also known as C-TAK1 or PAR-1A, is a serine/threonine kinase that plays essential roles in cell polarity, microtubule dynamics, and cell cycle regulation. As a member of the MAP/microtubule affinity-regulating kinase family, MARK3 phosphorylates microtubule-associated proteins and participates in the regulation of Cdc25C, positioning it as a significant target for researchers investigating signal transduction pathways, cytoskeletal organization, and cell division control mechanisms.

This recombinant monoclonal antibody, clone 5C7, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthesized peptide from human MARK3 protein and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Affinity chromatography purification ensures high purity for reliable performance across experiments.

Validation studies demonstrate robust performance in immunohistochemistry, where staining at 1:100 dilution successfully detected MARK3 in paraffin-embedded human small intestine tissue and human cervical cancer samples using citrate buffer antigen retrieval. Flow cytometry applications have been validated using HeLa cells, with clear positive signal separation from isotype control when cells were fixed and permeabilized, confirming detection of intracellular MARK3. These validated applications provide flexibility for researchers working with both tissue sections and cell-based assays.

The non-conjugated format allows pairing with your preferred secondary detection system, while the glycerol-containing buffer formulation supports long-term storage stability. For investigators exploring MARK3 function in polarity establishment, cytoskeletal regulation, or oncogenic signaling pathways, this antibody delivers the technical reliability needed to advance your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.

Gene References into Functions

Authors identified a regulatory switch controlled by MARK3 that couples microtubules to the actin cytoskeleton to establish epithelial cell polarity through ARHGEF2. PMID: 29089450
findings indicate that brain PP-1I associates with and is regulated by the associated protein kinases C-TAK1 and PFTK1 PMID: 25028520
We have shown that serum ALP levels modified the association of MARK3 with bone mineral density in osetoporosis for Chinese patients. PMID: 23894155
interaction between Mitf and C-TAK1 was demonstrated. PMID: 20214879
results show that LKB1 can direct the phosphorylation of the serine-threonine kinase PAR1A PMID: 12879020
identify sequence motifs required for stable C-TAK1 association and substrate phosphorylation PMID: 12941695
The C-TAK1/p78 constitutively associates with and phosphorylates KSR1 generating a 14-3-3 binding site. PMID: 15182702
Data show that the protein kinase Cdc25 C-associated kinase 1 (C-TAK1) is a binding partner and a substrate of Pim-1, and suggest a role for Pim-1 as a positive regulator at the G(2)/M transition of the cell cycle. PMID: 15319445
14-3-3 binding to MARK3 is dependent on phosphorylation. PMID: 16968750
Class IIa histone deacetylases (HDACs) are subjected to signal-independent nuclear export that relies on their constitutive phosphorylation. EMK and C-TAK1, are identified as regulators of this process. PMID: 16980613
identify a molecular mechanism through which the hMARK3 UBA domain has evolved to bind the kinase domain PMID: 17726107
occurrence of an unusual TG 3' splice site in intron 3 PMID: 17672918

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Subcellular Location

Cell membrane; Peripheral membrane protein. Cell projection, dendrite. Cytoplasm.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 6897

OMIM: 602678

KEGG: hsa:4140

STRING: 9606.ENSP00000411397

UniGene: Hs.35828

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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