Product Details

Uniprot No.

Target Names

Alternative Names

CDC 46 antibody; CDC46 antibody; CDC46 homolog antibody; Cell division cycle 46 antibody; DNA replication licensing factor antibody; DNA replication licensing factor MCM 5 antibody; DNA replication licensing factor MCM5 antibody; MCM 5 antibody; MCM5 antibody; MCM5_HUMAN antibody; MGC5315 antibody; Minichromosome maintenance complex component 5 antibody; Minichromosome maintenance deficient (S. cerevisiae) 5 antibody; Minichromosome maintenance deficient 5 antibody; Minichromosome maintenance deficient protein 5 antibody; P1 CDC46 antibody; P1-CDC46 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human MCM5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MCM5 serves as an essential component of the minichromosome maintenance complex, functioning as a DNA replication licensing factor that ensures accurate genome duplication occurs only once per cell cycle. This protein plays a critical role in the initiation of DNA replication and is frequently studied in the context of cell proliferation, cancer biology, and cell cycle regulation research.

This recombinant monoclonal antibody, clone 17B7, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced through controlled expression systems, you can expect consistent performance across different lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The rabbit host origin combined with affinity chromatography purification yields a highly specific reagent suitable for demanding experimental workflows.

Validation studies demonstrate robust performance in flow cytometry applications using human Raji cells. In these experiments, cells were fixed with formaldehyde and permeabilized with Triton X-100, followed by blocking with normal goat serum. The antibody produced clear positive signal separation from isotype control, with recommended working dilutions ranging from 1:50 to 1:200 for flow cytometry applications. The antibody has also been validated for ELISA, providing flexibility for researchers who need to assess MCM5 levels across different experimental platforms.

The unconjugated format allows you to pair this antibody with your preferred secondary detection system, accommodating various fluorophore or enzyme conjugates depending on your instrumentation and multiplexing requirements. This MCM5 antibody is particularly well-suited for investigations into DNA replication dynamics, cell cycle checkpoint mechanisms, and proliferative states in cancer research models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Gene References into Functions

Minichromosome maintenance 5 was shown as an independent prognostic biomarker for patients with cervical cancer PMID: 29642758
MCM5 expression is associated with the malignant status and poor prognosis in cervical adenocarcinoma patients. PMID: 29253764
The RAS-related nuclear protein ((P) ran), breast cancer metastasis suppressor 1 ((P) brms1) and minichromosome maintenance complex component 5 ((P) mcm5) promoters have the specificity and strength needed for cancer-specific expression-targeted gene therapy. PMID: 27140445
the incorporation of physiological levels of MCM5 into HIV-1 virions facilitates viral cDNA accumulation in newly infected cells, probably by modifying nucleic acid configuration during reverse transcription. PMID: 27414250
MCM5 is a novel gene involved in Meier-Gorlin syndrome. PMID: 28198391
Our findings support the hypothesis that MCM5 targeting may be regarded as an effective molecular therapy against anaplastic thyroid carcinoma PMID: 26911376
we could consider miRNA-10b and MCM5 mRNA as prognostic markers and potential therapeutic targets in breast cancer to be applied to other patient data sets. PMID: 25596707
The increased expression of MCM5 protein begins at the oral pre-cancerous stage and is significantly associated with the aggressive progression and poor prognosis of OSCC. PMID: 24245508
Data indicate that MCM-BP binds USP7 on chromatin and can mediate an interaction between the USP7 and MCM proteins. PMID: 24190967
The results of this study demonistrated that Mcm5 shows a highly significant correlation with Alcohol use disorders-induced decreases in the volume of the left parietal supramarginal gyrus and neuropsychological measures. PMID: 23095216
MicroRNA miR-885-5p targets CDK2 and MCM5, activates p53 and inhibits proliferation and survival. PMID: 21233845
lack of MCM5 proteins expression which may explain commonly known low mitotic activity of desmoid tumour cells PMID: 21478101
MCM-5 expression was significantly associated with tumor size, presence of lymph node metastases and tumor histopathological stage. Patients with high MCM-5 expression had significantly shorter survival times. PMID: 20694513
Could use the urinary hTERT, SENP1, PPP1CA, and MCM5 mRNA to detect bladder cancer recurrence. PMID: 21106093
Results further confirm the importance of MCM5 in malignant transformation and in the pathogenesis of cervical dysplasia. PMID: 15696126
The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation. PMID: 16199513
MCM5 is part of the GINS complex. PMID: 17557111
results demonstrate pattern and frequency of MCM5 expression in various skin diseases PMID: 17988337
Immunofluorescence technology to quantify cervical cell expression of two biomarkers p16(INK4A) and Mcm5 was developed and evaluated by both microscopy and flow cytometry. PMID: 18181189
No significant association was observed between MCM-5 protein expression and the clinicopathological characteristics of colon cancer examined. PMID: 18465232
Cyclin E is shown to directly interact with and colocalize on centrosomes with the DNA replication factor MCM5 in a centrosomal localization sequence-dependent but Cdk2-independent manner. PMID: 18799789
Analysis of MCM5 expression in aggressive fibromatosis (desmoid tumor) is reported. PMID: 19130399

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Involvement in disease

Meier-Gorlin syndrome 8 (MGORS8)

Subcellular Location

Nucleus. Chromosome. Cytoplasm, cytosol.

Protein Families

MCM family

Database Links

HGNC: 6948

OMIM: 602696

KEGG: hsa:4174

STRING: 9606.ENSP00000216122

UniGene: Hs.517582

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Code	CSB-RA062698A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing Raji cells stained with CSB-RA062698A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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MCM5 Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background