METAP2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA253256A0HU
Size	US$210
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Image	Flow cytometric analysis of methionyl aminopeptidase 2 expression in HepG2 cells using methionyl aminopeptidase 2 antibody. Green, isotype control; red, methionyl aminopeptidase 2. Immunocytochemical staining of HepG2 cells with Methionyl aminopeptidase 2 antibody. Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using methionyl aminopeptidase 2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm. Western blotting analysis using methionyl aminopeptidase 2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with methionyl aminopeptidase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P50579

Target Names

METAP2

Alternative Names

METAP2; Methionyl Aminopeptidase 2; MNPEP; MAP2; P67; Initiation Factor 2-Associated 67 KDa Glycoprotein; Methionine Aminopeptidase 2; Peptidase M; P67eIF2; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Peptidase M 2; EC 3.4.11.18; P67EIF2; MetAP 2; MAP 2

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human METAP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, FC, ICC, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

Gene References into Functions

MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
Expressed in colon cancer cells PMID: 15336565
MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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Subcellular Location

Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.

Protein Families

Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily

Database Links

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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