Code | CSB-RA982003A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:50-1:200 |
FC | 1:50-1:200 |
In efforts to create a recombinant monoclonal antibody specific to NEUROD1, the initial step involved immunizing a rabbit with a synthesized peptide derived from human NEUROD1 protein. Following immunization, B cells were isolated from the rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which served as a template for extending NEUROD1 antibody genes using degenerate primers. These extended NEUROD1 antibody genes were incorporated into a plasmid vector and introduced into host cells for expression. Subsequently, the NEUROD1 recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and evaluated for its utility in ELISA, IF, and FC applications, showing specific reactivity with human NEUROD1 protein.
NEUROD1 is a critical transcription factor that orchestrates the differentiation and maturation of neurons during development, contributes to the formation of neural circuits, and plays a role in the maintenance of neuronal identity. Its functions are essential for the proper functioning of the nervous system, and its dysregulation can have significant implications for neurological health and disease.
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