Code | CSB-RA898300A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
The NQO2 recombinant monoclonal antibody was produced through a series of precise steps: Immunization and B cell isolation: An immunized animal's spleen was used to isolate B cells. The immunogen used during the immunization process was a synthesized peptide derived from human NQO2. RNA extraction and cDNA synthesis: RNA was extracted from the isolated B cells, and reverse transcription was performed to convert the RNA into cDNA. Amplification and vector construction: The gene encoding the NQO2 antibody was amplified using a degenerate primer, and the amplified gene was inserted into a vector, creating a construct for antibody expression. Transfection and antibody expression: The recombinant vector was introduced into host cells through transfection, allowing for the expression of the NQO2 recombinant monoclonal antibodies. Antibody harvesting and purification: The NQO2 recombinant monoclonal antibodies were harvested from the cell culture supernatant and subsequently purified using affinity chromatography. This purification step ensured the isolation of high-quality antibodies. Antibody validation: This NQO2 recombinant monoclonal antibody can be used in the ELISA and WB for the detection of human and mouse NQO2 proteins.
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