NQO2 Antibody

Code CSB-PA016040LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: K562 whole cell lysate, Mouse liver tissue
    All lanes: NQO2 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA016040LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA016040LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunoprecipitating NQO2 in K562 whole cell lysate
    Lane 1: Rabbit control IgG (1ug) instead of CSB-PA016040LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA016040LA01HU (8ug) + K562 whole cell lysate (500ug)
    Lane 3: K562 whole cell lysate (10ug)

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue, Mouse Kidney tissue
    All lanes: NQO2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 26 kDa
    Observed band size: 26 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NQO2 Polyclonal antibody
Uniprot No.
Target Names
NQO2
Alternative Names
DHQV antibody; DIA6 antibody; EC 1.10.99.2 antibody; MGC94180 antibody; NAD(P)H dehydrogenase quinone 2 antibody; NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody; NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody; NMOR2 antibody; NQO 2 antibody; NQO2 antibody; NQO2_HUMAN antibody; NRH dehydrogenase [quinone] 2 antibody; NRH dehydrogenase antibody; NRH:quinone oxidoreductase 2 antibody; OTTHUMP00000015948 antibody; OTTHUMP00000015949 antibody; OTTHUMP00000015953 antibody; Ox 2 antibody; Ox2 antibody; QR2 antibody; Quinone antibody; Quinone reductase 2 antibody; Ribosyldihydronicotinamide dehydrogenase [quinone] antibody; Ribosyldihydronicotinamide dehydrogenase antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human Ribosyldihydronicotinamide dehydrogenase [quinone] protein (1-231AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The NQO2 Antibody (Product code: CSB-PA016040LA01HU) is Non-conjugated. For NQO2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA016040LB01HU NQO2 Antibody, HRP conjugated ELISA
FITC CSB-PA016040LC01HU NQO2 Antibody, FITC conjugated
Biotin CSB-PA016040LD01HU NQO2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

To prepare the NQO2 polyclonal antibody, the recombinant human NQO2 protein (1-231aa) is used to immunize a rabbit. The rabbit's immune system will recognize the immunogen as foreign and produce antibodies against it. The NQO2 polyclonal antibodies are purified from the rabbit's serum using protein G affinity chromatography. The purity of this NQO2 antibody is over 95%. This NQO2 antibody can be used to detect human and mouse NQO2 proteins in multiple applications, including ELISA, WB, IHC, IF, and IP.

NQO2 protein mainly functions to catalyze the reduction of quinones, which are harmful compounds that can cause oxidative damage to cells. NQO2 also plays a role in the metabolism of drugs, toxins, and carcinogens, and is involved in other cellular processes such as regulation of gene expression, cell proliferation, and apoptosis. Mutations in the NQO2 gene have been linked to several diseases, such as cancer and Parkinson's disease.

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Target Background

Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Gene References into Functions
  1. The ontogeny and population variability of human hepatic dihydronicotinamide riboside:quinone oxidoreductase (NQO2). PMID: 28346733
  2. In patients with Alzheimer's disease, QR2 is overexpressed in the insular cotex. PMID: 26609153
  3. NQO2-L47 is less stable towards proteolytic digestion and thermal denaturation than NQO2-F47. PMID: 24631540
  4. NQO1 609C>T and NQO2 -3423G>A polymorphisms do not seem to play any significant role in susceptibility or prognosis of EC in north Indian population. PMID: 22770696
  5. This first structure of a reduced quinone reductase shows that reduction of the FAD cofactor and binding of a specific inhibitor lead to global changes in NQO2 structure and is consistent with a functional role for NQO2 as a flavin redox switch. PMID: 23471972
  6. both NQO1 and NQO2 modulate the efficacy of AC therapy and that NQO2 is associated with tamoxifen toxicity. PMID: 21946896
  7. results indicate a role of NQO2 in the control of AKT/GSK-3beta/cyclin D1 and highlight the involvement of NQO2 in degradation of cyclin D1, as part of mechanism of chemoprevention by resveratrol PMID: 22266466
  8. Results suggest that NQO2, SOD2 and SOD3 may significantly modify prognosis of breast cancer patients. PMID: 21351093
  9. results suggest that the increase in hippocampal QR2 might be a cause of AD or might promote the progression of AD by causing an increase in the toxic quinone levels and consequent loss of cognitive function. PMID: 21803122
  10. Selective inhibition of NQO2 over NQO1 can be attributed to the bulky aminoalkylamino substituent at the 5-position, which blocks entrance of the indolequinones into the NQO1 active site. PMID: 21718050
  11. NQO2 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
  12. The NQO1 609C>T TT genotype and T allele were significantly associated with increased risk for gastric cancer (GC), whereas NQO2 -3423G>A polymorphism did not show any association with GC. PMID: 21294640
  13. The first European study of the SOD2, SOD3, NQO1, and NQO2 roles in pancreatic cancer etiology did not find significant associations. PMID: 20966810
  14. We observed a nominal significant association between the rs1143684 NQO2 polymorphism and the trajectory of delayed memory recall over time (p=0.029). No other associations were seen with the decline of other cognitive abilities. PMID: 18538895
  15. Our findings challenge the previous assumption that the NQo2 I-16 allele is stable only when paired with another I-16 allele PMID: 19495956
  16. polymorphisms associated with idiopathic Parkinson's disease PMID: 11688992
  17. A significant difference was found between alcoholic patients and controls in genotype frequency at an insertion/deletion site in the promoter region of the NQO2 gene (p = 0.0014). PMID: 12960511
  18. the level of NQO2 mRNA is low in AGR patients compared with the control group. Such a reduction in message suggests that the NQO2 gene may be involved in the development of clozapine-induced AGR. PMID: 14617031
  19. NQO2 gene may confer susceptibility to a certain form of schizophrenia. PMID: 14639047
  20. the NQO2 gene is differentially expressed by the polymorphic promoters in human cells transfected with NQO2 gene reporter constructs. Alterations in NQO2 activity might be an important factor in susceptibility to Parkinson's disease. PMID: 15451063
  21. We propose that induction of NQO2 may relate to the observed increased expression of p53 that, in turn, contributes to the observed suppression of cell growth in both melanoma cell lines. PMID: 15993843
  22. the nicotinamide adenine dinucleotide phosphate reduced-diaphorase interneurons in the human striatum. PMID: 16025450
  23. The results combined demonstrated that Nrf2 associates with JunD, binds to ARE at nucleotide -1433, and regulates human NQO2 gene expression and induction in response to antioxidants. PMID: 16545679
  24. an apparent association between an NQO2 exon 3 single-nucleotide polymorphism and lower enzymatic activity PMID: 17332305
  25. The crystal structure of melatonin and 2-iodomelatonin in complex with QR2 provide a detailed description of the enzyme active site; the design of inhibitors of the enzyme might be instrumental in discriminating the role of MT1, MT2 and QR2/MT3 PMID: 18254726
  26. Parkinson's disease patients were analyzed for NQO2 gene promoter polymorphisms. Three allelic variants were I-29, I-16 and D. The association of the D promoter with Parkinson's disease may be due to an increase in expression of the NQO2 gene. PMID: 18314446
  27. This first structural analysis of hQR2 should enable to better understand the biological role of melatonin on this enzyme . PMID: 18502195
  28. Quinone reductase 2 is a catechol quinone reductase. quinone reductase 2 could play important roles in the regulation of catecholamine oxidation processes that may be involved in the etiology of Parkinson disease. PMID: 18579530
  29. nmr studies of QR2 catalytic reaction were performed; results led to conclusion that melatonin is not cleaved to form N1-acetyl-N2-formyl-5-methoxykynurenine by a catalytically active QR2, indicating melatonin is neither a substrate nor a co-substrate PMID: 18826489
  30. NQO2 is a susceptibility gene for breast carcinogenesis. PMID: 19351655

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Subcellular Location
Cytoplasm.
Protein Families
NAD(P)H dehydrogenase (quinone) family
Database Links

HGNC: 7856

OMIM: 160998

KEGG: hsa:4835

STRING: 9606.ENSP00000337773

UniGene: Hs.533050

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