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NRF1 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA219102A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Raji whole cell lysate(30µg), K562 whole cell lysate(30µg), HeLa whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg)
    All lanes: NRF1 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54 kDa
    Observed band size: 68 kDa
    Exposure time: 5min
  • Overlay Peak curve showing HEK293T cells stained with CSB-RA219102A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q16656
Target Names
NRF1
Alternative Names
alpha pal antibody; alpha palindromic binding protein antibody; Alpha palindromic-binding protein antibody; Alpha-pal antibody; locus control region factor 1 antibody; NFE2 related factor 1 antibody; NRF-1 antibody; Nrf1 antibody; NRF1_HUMAN antibody; Nuclear respiratory factor 1 antibody
Species Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human NRF1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
14B4
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

NRF1, or Nuclear Respiratory Factor 1, serves as a master transcriptional regulator coordinating the expression of nuclear-encoded mitochondrial genes essential for oxidative phosphorylation, mitochondrial DNA replication, and cellular energy metabolism. This transcription factor plays a central role in mitochondrial biogenesis and has emerged as a key focus in research spanning metabolic disorders, neurodegeneration, cancer metabolism, and aging-related cellular dysfunction.

This recombinant monoclonal antibody, clone 14B4, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human NRF1 and purified by affinity chromatography, ensuring high specificity for your target.

Validation across multiple platforms demonstrates this antibody's experimental versatility. Western blot analysis successfully detects NRF1 across diverse human cell lines including Raji, K562, HeLa, COLO205, and HepG2, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. The observed band at 68 kDa, rather than the predicted 54 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation or glycosylation commonly associated with transcription factors. Flow cytometry validation in HEK293T cells demonstrates clear positive signal separation from isotype controls, supporting intracellular detection applications.

Whether investigating mitochondrial dysfunction in disease models, exploring metabolic reprogramming in cancer cells, or characterizing energy homeostasis pathways, this NRF1 antibody provides reliable detection across western blotting and flow cytometry applications with validated performance in both human and mouse experimental systems.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Gene References into Functions
  1. CB-5083 decreases viability in multiple myeloma cell lines and patient-derived multiple myeloma cells, including those with background proteasome inhibitor (PI) resistance. CB-5083 has a unique mechanism of action that combines well with PIs, which is likely owing to the p97-dependent retro-translocation of the transcription factor, Nrf1, which transcribes proteasome subunit genes following exposure to a PI PMID: 28878026
  2. Chronic kidney disease patients undergoing hemodialysis might be subjected to potential mitochondrial oxidative dysregulation and NRF1 down-regulation. PMID: 28164673
  3. this study therefore identifies NRF-1 as a novel regulator of HIF-1a. PMID: 27491637
  4. NRF-1 and its target mitochondrial transcription factor A (TFAM) were higher in Tamoxifen (TAM)-resistant LCC2 and LCC9 cells than TAM-sensitive MCF-7 cells. PMID: 27515002
  5. NRF1 overexpression attenuated BPDEinduced Sphase arrest via the ATM/Chk2 signaling pathway. PMID: 27035420
  6. Low NRF1 expression was associated lymphatic metastasis and radio-resistance in nasopharyngeal carcinoma. PMID: 26201446
  7. EglN2 associates with the NRF1-PGC1alpha complex and controls mitochondrial function in breast cancer PMID: 26492917
  8. We show that composite nucleosomes containing mH2A and NRF-1 are stably positioned on gene regulatory regions and can buffer transcriptional noise associated with antiviral responses PMID: 25959814
  9. Results present evidence in the support of E2-induced ROS-mediated AKT signalling leading to the activation of NRF-1-regulated cell cycle genes. PMID: 25965299
  10. This study identified a regulatory branch of the mitochondrial unfolded protein response (UPR(mt)), which is mediated by the interplay of SIRT7 and NRF1 and is coupled to cellular energy metabolism and proliferation. PMID: 25792330
  11. The mitochondria targeting sequence-deficient hTFAM also repressed Tfam promoter activity to the same degree as hTFAM. PMID: 24875355
  12. Low NRF1 expression is associated with chronic kidney disease after dialysis. PMID: 24204994
  13. We verified that NRF-1 positively regulates FAM134C and ENOX1, and negatively regulates C3orf10 in human neuroblastoma IMR-32 cells and primary rat cortical neurons. PMID: 23939472
  14. NRF-1 regulates Atp1a1 and Atp1b1 and are important in mediating the energy generation and neuronal activity. PMID: 23048038
  15. APE1 is a new coactivator of NRF1, demonstrating an additional regulatory role of APE1 in maintenance of mitochondrial functionality. PMID: 22580151
  16. Given that NRF-1 is a key nuclear transcription factor regulating genes involved in mitochondrial activity and biogenesis, these data suggest that diesel exhaust particulate extracts may adversely affect mitochondrial function. PMID: 22105178
  17. Single nucleotide polymorphism in NRF1 gene is associated with epithelial ovarian cancer. PMID: 21447778
  18. Upon IMS stress, overproduction of reactive oxygen species (ROS) and phosphorylation of AKT triggers estrogen receptor (ER) activity, which further upregulates the transcription of the mitochondrial regulator NRF1 and the IMS protease HTRA2. PMID: 21486948
  19. Both NRF-1 and GABP have been linked to the regulation of nuclear-encoded mitochondrial proteins, and the results of this study suggest their expression is coordinated by NRF-1's activation of the GABPbeta promoter. PMID: 21609478
  20. Data demonstrated that the polymorphism in TP53 (rs1042522) was associated with type 2 diabetes, and that potential interaction of TP53 (rs1042522) and RAPGEF1 (rs11243444), or NRF1 (rs1882095) increased the risk of type 2 diabetes. PMID: 21146886
  21. Findings indicate that TLR4-dependent NFkappaB and CREB activation co-regulate the NRF1 promoter with NFkappaB intronic enhancement and redox-regulated nuclear translocation, leading to downstream target-gene expression. PMID: 20587593
  22. Taken together, these results confirm the direct interaction of NRF-1 and ERR alpha with PGC-1 beta, and their participation in mitochondrial biogenesis and respiration. PMID: 20561910
  23. the obligatory intestinal folate transporter PCFT (SLC46A1) is regulated by nuclear respiratory factor 1 PMID: 20724482
  24. This study demonstrates that NRF-1 is a novel transcriptional inhibitor of the human ACCbeta gene promoter in the mammalian heart. PMID: 20599696
  25. Effect of gene on proliferation arrest in a non-small cell bronchopulmonary cancer line. PMID: 12174908
  26. Insulin resistance and DM associate with reduced expression of multiple nuclear respiratory factor-1 (NRF-1)-dependent genes encoding key enzymes in oxidative metabolism and mitochondrial function PMID: 12832613
  27. Overexpression of human nuclear respiratory factor 1 in transgenic mice increases glucose transport capacity in skeletal muscle. PMID: 12958173
  28. alpha-Pal/NRF-1 is an essential transcription factor in the regulation of human IAP gene expression PMID: 14747477
  29. specifically binds the 21-bp minimal promoter and positively contributes to transcription of the X gene of Hepatitis B virus. PMID: 15452206
  30. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene. PMID: 15992771
  31. There is an association between NRF-1 genotypes (rs2402970 and rs6949152 polymorphisms) and the baseline and/or training response of human aerobic capacity. PMID: 18184751
  32. These findings support a role for MEF2A as an intermediary in coordinating respiratory chain subunit expression in heart and muscle through a NRF1 --> MEF2A --> COX(H) transcriptional cascade. PMID: 18222924
  33. results reveal that especially NRF-1, along with AP-1 and, to a minor extent, an Sp1 site, is essential for human CAPNS1 promoter activity and gene expression PMID: 18234454
  34. Data show that NRF-1 is the main transcription factor regulating the expression of TOMM34, and that Sp1 interacts with NRF-1 to stimulate the promoter's full activity. PMID: 18364745
  35. No differences in mRNA levels of genes which mediate the transcriptional of mitochondrial biogenesis (PPARGC1A and NRF1) or in mitochondrial mass between diabetic and control myotubes. PMID: 18719883
  36. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665
  37. Deletion and site-directed mutagenesis combined with in silico transcription factor binding analysis of VSNL1 promoter identified nuclear respiratory factor (NRF)-1/alpha-PAL as a major player in regulating VSNL1 minimal promoter activity. PMID: 19674972
  38. Fig 2B describes a putative bZIP motif; however, this peptide sequence has no sequence similarity to the conserved smart00338:BRLZ or pfam03131:bZIP_Maf protein domains. PMID: 8034649
  39. The authors incorrectly use the symbol NFE2L1; this publication discusses nuclear respiratory factor 1 which has an official symbol of NRF1. PMID: 7558044

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Subcellular Location
Nucleus.
Protein Families
NRF1/Ewg family
Tissue Specificity
Ubiquitously expressed with strongest expression in skeletal muscle.
Database Links

HGNC: 7996

OMIM: 600879

KEGG: hsa:4899

STRING: 9606.ENSP00000223190

UniGene: Hs.654363

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