OS9 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA959292A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HL-60 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue All lanes: OS9 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 70, 68, 74, 66, 64, 70, 61 kDa Observed band size: 72-95 kDa IHC image of CSB-RA959292A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q13438

Target Names

OS9

Alternative Names

Protein OS-9 (Amplified in osteosarcoma 9), OS9

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human OS9

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

26H3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the OS9 recombinant monoclonal antibody follows a meticulous and standardized process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, with the synthesized peptide derived from human OS9 used as the immunogen. Total RNA is extracted from the B cells and converted into cDNA through reverse transcription. The OS9 antibody genes are then amplified using PCR with primers specific to the antibody constant regions and inserted into an expression vector. The vector is introduced into host cells, allowing for the production of the OS9 recombinant monoclonal antibody. After harvesting the antibody from the cell culture supernatant, it undergoes purification using affinity chromatography to obtain a highly purified form. Rigorous characterization assays, including ELISA, WB, and IHC analysis, are performed to validate the antibody's specificity and functionality, ensuring its precise binding to human and mouse OS9 protein. This meticulous production process ensures the generation of a reliable and effective OS9 recombinant monoclonal antibody, suitable for various applications in OS9-related research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.

Gene References into Functions

The endoplasmic reticulum-associated protein, OS-9, behaves as a lectin in targeting the immature calcium-sensing receptor. PMID: 28419469
OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition. PMID: 25999789
EDEM2 and OS-9 are required for ER-associated degradation of non-glycosylated sonic hedgehog PMID: 24910992
Unique regions mediate the interaction between OS-9 and GRP94. PMID: 24899641
It delivers mutant neuroserpin to ERAD by recognition of glycan side chains and provide the first in vivo proof of involvement of ERAD in degradation of mutant neuroserpin. PMID: 24795221
long non-coding RNA ENST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1alpha in pancreatic ductal adenocarcinoma. PMID: 25314054
Data indicate that the interaction of OS-9 and XTP3-B with CD147(CG) was inhibited by mutations to conserved residues in their lectin domains. PMID: 23097496
OS-9 plays no direct functional role in HIF degradation since physical interaction of OS-9 with oxygen sensing HIF prolyl hydroxylases cannot occur in vivo due to their different subcellular localization PMID: 21559462
It regulates endoplasmic reticulum-associated degradation. (review) PMID: 21404621
The OS-9 specifically recognizes Manalpha1,6Manalpha1,6Man residues on the processed C-arm through the continuous double tryptophan (WW) motif. PMID: 21172656
The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation PMID: 19914915
Identification of a peptide, resulting from a point mutation in gene OS-9, recognized by cytolytic T lymphocytes on a human melanoma. PMID: 12747754
OS-9 regulates the secretory transport of TRPV4 and appears to protect TRPV4 subunits from the precocious ubiquitination and ER-associated degradation. PMID: 17932042
OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD PMID: 18264092
Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum inhibit secretion of misfolded protein conformers and enhancing their disposal PMID: 18417469
OS9 is critically involved in the modulation of ER-to-Golgi transport of DC-STAMP in response to TLR triggering, suggesting a novel role for OS9 in myeloid differentiation and cell fusion. PMID: 18952287
OS-9 recognises terminally misfolded proteins via polypeptide-based rather than glycan-based signals, but is only required for transferring those bearing N-glycans to the ubiquitination machinery. PMID: 19084021
model for mannose trimming and the requirement for OS-9 lectin activity in glycoprotein ERAD in which N-glycans lacking the terminal mannose from the C branch are recognized by OS-9 and targeted for degradation PMID: 19346256

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Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

OS-9 family

Tissue Specificity

Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.

Database Links

HGNC: 16994

OMIM: 609677

KEGG: hsa:10956

STRING: 9606.ENSP00000318165

UniGene: Hs.527861

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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