Product Details

Uniprot No.

Target Names

Alternative Names

CALM antibody; Clathrin assembly lymphoid myeloid leukemia antibody; Clathrin assembly lymphoid myeloid leukemia protein antibody; CLTH antibody; LAP antibody; Phosphatidylinositol-binding clathrin assembly protein antibody; PICAL_HUMAN antibody; PICALM antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PICALM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PICALM (phosphatidylinositol-binding clathrin assembly protein) plays a central role in clathrin-mediated endocytosis, facilitating the internalization of membrane receptors and regulating intracellular trafficking. This protein has garnered significant attention in neuroscience research due to its genetic association with Alzheimer's disease risk, where variants in the PICALM locus have been consistently identified in genome-wide association studies. Understanding PICALM expression and function is therefore critical for researchers investigating endocytic pathways, synaptic vesicle recycling, and neurodegenerative disease mechanisms.

This recombinant monoclonal antibody, clone 7F10, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide from human PICALM and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your results remain comparable across extended studies.

Validation across multiple applications demonstrates genuine experimental flexibility. Western blot analysis confirms robust detection in human cell lines including HT-29, HeLa, HEK293, and MCF-7, with observed bands at 69 and 71 kDa closely matching the predicted 71 kDa molecular weight. The slight size variation likely reflects post-translational modifications or alternative isoform expression. Immunohistochemistry has been validated in paraffin-embedded human placenta tissue using standard citrate buffer antigen retrieval, while flow cytometry analysis in 786-O cells demonstrates clear positive signal separation from isotype controls.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability. Whether you are characterizing PICALM expression patterns, investigating endocytic machinery, or exploring its role in neurological disease models, this antibody provides a reliable tool for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).

Gene References into Functions

IN PICALM rs3851179 polymorphism, the age- dependent increase in the P3 latency was more pronounced in the PICALM GG carriers than in the carriers of the PICALM AA and PICALM AG genotypes. PMID: 30472946
There were significant differences in genotype and allele frequencies for the SNP PICALM rs3851179 between Brazilian Alzheimer and Parkinson disease cases and controls, but none for CR1 rs6656401 and CLU rs11136000 intronic polymorphisms. PMID: 28567584
The PICALM rs3851179 polymorphism significantly affects the default mode network network in mild cognitive impairment PMID: 28549650
No association for PICALM with Alzheimer's disease in south-Indian population. PMID: 28558900
this updated meta-analysis highlights the involvement of PICALM rs3851179 variant in Alzheimer's disease susceptibility in Chinese population. PMID: 27048444
suggest that there is a close relationship between abnormal PICALM processing, tau pathology and impairment of autophagy in human neurodegenerative diseases PMID: 27260836
There was no significant association between single nucleotide polymorphisms (SNPS) of GAB2 rs2373115 (G > T) and PICALM rs541458 (C > T) and Alzheimer's disease (AD). The allele T of rs3851179 in PICALM was associated with a 13 % increase in the risk of AD. Seven SNPs on SORL1 were significantly associated with AD. PMID: 26611835
Hippocampal shape features derived from the diffeomorphic metric-based shape analysis led to the identification of significant CLU-PICALM interaction effects on hippocampal morphology in young healthy adults, which were not identified by volume measurement and voxel-wise analysis. PMID: 27017968
This study provided preliminary evidences supporting that PICALM variations render protections by facilitating reserve capacities of posterior cingulate in non-demented elderly. PMID: 27117083
rs3851179 in PICALM was associated with an increased risk of gestational diabetes. The frequency of the Alzheimer's disease risk-associated C allele was significantly higher in the gestational diabetes group compared to controls. The C allele of the PICALM SNP was protective for impaired glucose tolerance. PMID: 28316001
Alzheimer's disease risk PICALM GG genotype suggests changes in the cortical excitatory-inhibitory balance, which are heightened during normal aging. PMID: 28073596
Study concludes that the novel clathrin interaction sites identified here in CALM and AP180 have a major role in how these proteins interface with clathrin. This work advances the case that AP180 and CALM are required to use a combination of standard clathrin N-terminal domain binding motifs and the sequence identified here for optimal binding and assembling clathrin. PMID: 27574975
Depletion of PICALM in brain-derived cells has significant effects on the processing of APP, probably by reducing clathrin-mediated endocytosis. In particular, it affects the production of beta-C-terminal fragment which is increasingly considered to be an important mediator in Alzheimer's disease independent of Abeta. PMID: 27430330
reduction of CALM decreases Ab deposition as well as brain levels of insoluble Ab42 in vivo These results suggest that CALM expression modifies Alzheimer disease (AD)risk by regulating Aamyloid-beta protein pathology PMID: 27466196
PICALM is involved with late onset Alzheimer's disease. [review] PMID: 27773727
Our finding suggests that PICALM rs3851179 could contribute to cognitive impairment in older patients with Parkinson disease PMID: 26889634
PICALM and CLU risk genotypes exert differential impacts on the hippocampal resting-state functional connectivity in healthy young subjects. PMID: 24578178
PICALM has a role in modulating autophagy activity and tau accumulation PMID: 25241929
the rs541458 and rs3851179 of PICALM SNPs are not related to development of behavioral and psychological symptoms of dementia in Turkish population PMID: 26434199
We detected a significant association of the MTHFR rs1801133 and PICALM rs3851179 polymorphisms with AD. PMID: 25359311
PICALM SNP is significantly associated with late onset Alzheimer disease. PMID: 25022885
CALM is a major factor in controlling endocytic clathrin-coated vesicle size and maturation and hence in determining the rates of endocytic cargo uptake. PMID: 25898166
Data suggest a neural mechanism for APOE-PICALM interactions in patients with manifest Alzheimer's disease and indicate that the PICALM genotype modulates both brain atrophy and cognitive performance in APOE epsilon4 carrier PMID: 24613704
multiple PICALM isoforms are expressed in the human brain, that PICALM is robustly expressed in microvessels, and that expression of total PICALM is modestly correlated with the AD-associated SNP rs3851179. PMID: 24618820
allelic expression has shown that compelling PICALM allelic expression imbalance was not observed in most brain RNA samples PMID: 25169757
physical activity attenuated the effects of genetic risk (ie. the constellation of PICALM, BIN1, and CLU polymorphisms) on episodic memory PMID: 24660791
It might not play a major role in the genetic predisposition to Late-onset Alzheimer's disease in Han Chinese. PMID: 24095218
PICALM affects age at onset of Alzheimer's dementia in Down syndrome. PMID: 23601808
results confirm the association of PICALM gene with Alzheimer disease susceptibility in Korean population PMID: 22975751
We identified a mutation in CALM1 underlying IVF manifesting in childhood and adolescence. The causality of the mutation is supported by previous studies demonstrating that F90 mediates the direct interaction of CaM with target peptides. PMID: 24076290
This results suggest that besides APOE, PICALM associated with Alzheimer's disease risk do not exert large effects on the age-at-onset phenotype of Alzheimer disease. PMID: 23870418
CALM is able to sort VAMP4 and VAMP7, even though they have sorting signals for other clathrin adaptors. PMID: 23741335
The PICALM rs3851179 polymorphism is significantly associated with AD in the Asian population by both pooled analysis and meta-analysis methods. PMID: 23572399
AP-2/PICALM complex functions as an autophagic cargo receptor for the recognition and shipment of APP-CTF from the endocytic pathway to the LC3-marked autophagic degradation pathway. PMID: 24067654
Genome-wide association studies found 3 new susceptibility loci for Alzheimer disease(AD): complement receptor 1,clusterin,and the phosphatidylinositol-binding clathrin assembly protein. These SNPS are not associated with AD in a Polish population. PMID: 23650005
A CALM-derived nuclear export signal is essential for CALM-AF10-mediated leukemogenesis. PMID: 23487024
Our results indicate that rs3851179 may not be an AD susceptibility locus in the Chinese population and the APOEepsilon4-negative subgroup. PMID: 23040034
model system to study the effects of intracellular Abeta in fusion with green fluorescent protein. We sent this fusion protein into the secretory pathway and showed that intracellular traffic pathways are necessary for the generation of toxic species. PMID: 22888099
rs592297, a known coding synonymous SNP that is part of an exonic splice enhancer region in exon 5 of the PICALM gene, is in strong linkage disequilibrium with rs3851179. PMID: 22943764
Our results do not provide evidence that the PICALM rs3851179 polymorphism increase susceptibility to Parkinson disease, in the Greek population. PMID: 22715855
AP180 and CALM are endocytic adaptors dedicated to the sorting of small soluble N-ethylmaleimide-sensitive-factor attachment protein receptors. (Review) PMID: 22639918
CLU and CR1 were associated with more rapid cognitive decline. PICALM was associated with an earlier age at midpoint of cognitive decline. PMID: 22952074
In conclusion, we confirmed association of CLU, CR1, and PICALM genes with the disease status in our cohort through identification of a number of disease-specific variants PMID: 22402018
While no Alzheimer Disease association was observed with single nucleotide polymorphisms, a trend of association was seen with the Picalm and Clusterin single nucleotide polymorphisms. PMID: 20570404
PICALM, an adaptor protein involved in clathrin-mediated endocytosis, regulates APP internalization and subsequent Abeta generation. PICALM contributes to amyloid plaque load in brain likely via its effect on Abeta metabolism. PMID: 22539346
The SNP genotype pattern at the PICALM gene is associated with episodic memory. PMID: 22539578
Single nucleotide polymorphisms (SNPs) are not significantly different within PICALM sporadic Alzheimer disease patients and unrelated age- and sex-matched healthy controls of Han Chinese. PMID: 21358043
variants in BIN1, CLU, CR1 or PICALM are associated with changes in the CSF levels of biomarkers PMID: 21347408
findings showed evidence of CR1, CLU, and PICALM and late-onset Alzheimer's disease (LOAD) susceptibility in an independent southern Chinese population PMID: 22015308
AP-2 and epsin-1 are both required to promote efficient internalization of activated PAR1 and recognize discrete receptor sorting signals PMID: 21965661

Hide All

Involvement in disease

A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.

Subcellular Location

Cell membrane. Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus.

Protein Families

PICALM/SNAP91 family

Tissue Specificity

Expressed in all tissues examined.

Database Links

HGNC: 15514

OMIM: 603025

KEGG: hsa:8301

STRING: 9606.ENSP00000377015

UniGene: Hs.163893

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Code	CSB-RA177398A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg) All lanes: PICALM antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 71 kDa Observed band size: 69&71 kDa Exposure time：20s IHC image of CSB-RA177398A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA177398A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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PICALM Recombinant Monoclonal Antibody

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