PRKAR2A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA197122A0HU
Size	US$210
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Image	IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB. IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB. Immunofluorescence staining of Hela with CSB-RA197122A0HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 512-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF-7 cells stained with CSB-RA197122A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P13861

Target Names

PRKAR2A

Alternative Names

cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2 PRKAR2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human PRKAR2A

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15G8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PRKAR2A serves as the type II-alpha regulatory subunit of cAMP-dependent protein kinase, playing a central role in the cAMP signaling cascade that governs diverse cellular processes including metabolism, gene expression, and cell proliferation. As a key modulator of protein kinase A activity, PRKAR2A has drawn significant research interest for its involvement in cancer biology and metabolic regulation, where dysregulated cAMP signaling can drive pathological outcomes.

This recombinant monoclonal antibody, clone 15G8, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human PRKAR2A and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation, ensuring your experimental results remain comparable across studies and over time.

Validation across multiple detection platforms demonstrates this antibody's versatility in your workflow. Immunohistochemistry studies confirm reliable staining in paraffin-embedded human tissues, with clear detection observed in both normal kidney tissue and rectal cancer specimens at 1:50 dilution using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence analysis in HeLa cells reveals distinct staining patterns when counterstained with DAPI. Flow cytometry validation using MCF-7 breast cancer cells shows a clear positive shift compared to isotype control, confirming utility for quantitative single-cell analysis of PRKAR2A expression.

Supplied in a glycerol-containing buffer optimized for long-term stability, this antibody supports researchers investigating cAMP-dependent signaling networks, metabolic regulation, and oncogenic pathways where protein kinase A activity influences disease progression and therapeutic response.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Gene References into Functions

High PK-R2 expression is associated with colorectal cancer. PMID: 26372733
Prkar2a deficiency predisposes to hematopoietic malignancies in vivo. RIIalpha's likely association with HS and DLBCL was hitherto unrecognized and may lead to better understanding of these rare neoplasms. PMID: 26608815
Disruption of Snapin-PKR2 interaction did not affect PKR2 signaling, but increased the ligand-induced degradation, implying a role of Snapin in the trafficking of PKR2. PMID: 26687946
we demonstrate that neurochondrin has strong isoform selectivity towards the RIIa subunit of PKA with nanomolar affinity PMID: 25916936
These data demonstrate that some Kallmann syndrome-associated, intracellularly retained mutant PKR2 receptors can be functionally rescued, suggesting a potential treatment strategy for patients bearing such mutations. PMID: 24753254
Smad4 and the R subunit of the protein kinase A holoenzyme form a functional complex in vivo in response to TGFbeta. PMID: 23362281
ETO nervy homology region (NHR) 3 domain-PKA(RIIalpha) protein interaction does not appear to significantly contribute to AML1-ETO's ability to induce leukemia. PMID: 20708017
findings indicate that increased particulate type II protein kinase A activity occurs throughout pregnancy therefore directing the cAMP quiescence signal to specific subcellular loci within myometrial smooth muscle cells PMID: 12727975
These data implicate the involvement of PKA-RIIalpha anchoring apical targeting of distinct proteins and glycosphingolipids to apical plasma membrane domains and suggest that rerouting may underlie the delayed Golgi-to-apical surface transport of MDR1. PMID: 16723498
The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. PMID: 17081989
The data suggest that centrosomal anchoring of RIIalpha and the interrelated subapical positioning of these centrosomes is required for oncostatin M-, but not cAMP-mediated, bile canalicular lumen development. PMID: 17494870
RIIalpha releases Calpha upon elevated cAMP alone, dependent on autophosphorylation of the RIIalpha inhibitory domain PMID: 17884635
Bacillus anthracis edema toxin altered the protein levels and activity of protein kinase A and exchange protein activated by cAMP (Epac), a recently identified cAMP-binding molecule. PMID: 19307216

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Subcellular Location

Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane.

Protein Families

CAMP-dependent kinase regulatory chain family

Tissue Specificity

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Database Links

HGNC: 9391

OMIM: 176910

KEGG: hsa:5576

STRING: 9606.ENSP00000265563

UniGene: Hs.631923

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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