Code | CSB-RA176962A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
CUSABIO produced the PRMT5 recombinant monoclonal antibody using protein technology and DNA recombinant technology. The first step involved immunizing mice with a synthesized peptide derived from human PRMT5. After a period of time, the spleen cells were removed aseptically, and the total RNA was extracted and used to synthesize cDNA by RNA reverse transcription. The PRMT5 antibody gene was then amplified by PCR using the synthesized cDNA as a template. The PRMT5 antibody gene was then inserted into a vector and transfected into host cells for culturing. Finally, the PRMT5 recombinant monoclonal antibody was purified from the supernatant of cell culture using affinity chromatography and thoroughly verified for use in ELISA, WB, and IHC experiments for detecting human PRMT5 protein.
PRMT5 is an enzyme responsible for catalyzing the symmetric dimethylation of arginine residues in target proteins. PRMT5 has been shown to play important roles in cellular processes such as chromatin remodeling, transcriptional regulation, RNA splicing, and DNA damage repair. It has also been implicated in the development and progression of certain types of cancer.
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