PSMC5 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA879024A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: PC-3 whole cell lysate(20µg),HepG2 whole cell lysate(20µg), Jurkat whole cell lysate(20µg), U-251MG whole cell lysate(20µg),A549 whole cell lysate(20µg),MCF-7 whole cell lysate(20µg),HEK293 whole cell lysate(20µg),Mouse brain tissue lysate(20µg),Rat brain tissue lysate(20µg) All lanes: PSMC5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time：1min Immunofluorescence staining of Hela cell with CSB-RA879024A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA879024A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P62195

Target Names

PSMC5

Alternative Names

26S protease regulatory subunit 8 antibody; 26S proteasome AAA-ATPase subunit RPT6 antibody; Cim3 antibody; MSUG1 protein antibody; p45 antibody; p45/SUG antibody; Proteasome 26S ATPase subunit 5 antibody; Proteasome 26S subunit ATPase 5 antibody; Proteasome prosome macropain 26S subunit ATPase 5 antibody; Proteasome subunit p45 antibody; PRS8_HUMAN antibody; PSMC5 antibody; Rpt6 antibody; S8 antibody; SUG1 antibody; Tat binding protein homolog 10 antibody; TBP10 antibody; Thyroid hormone receptor interacting protein 1 antibody; Thyroid hormone receptor-interacting protein 1 antibody; Thyroid receptor interactor 1 antibody; TRIP1 antibody; TRIP1(SUG1) antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PSMC5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PSMC5, also known as RPT6 or SUG1, is an essential AAA-ATPase subunit of the 26S proteasome regulatory particle. This protein plays a critical role in ATP-dependent protein degradation through the ubiquitin-proteasome system, making it central to cellular processes including cell cycle regulation, signal transduction, and protein quality control. Beyond its proteasomal function, PSMC5 has been identified as a thyroid hormone receptor-interacting protein (TRIP1), suggesting additional roles in transcriptional regulation that continue to attract research interest.

This recombinant rabbit monoclonal antibody, clone 4E5, offers the reproducibility and consistency that demanding proteasome research requires. Developed against a synthetic peptide derived from human PSMC5, the recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple experimental platforms. In western blot applications, the antibody detects PSMC5 in a diverse panel of human cell lines including PC-3, HepG2, Jurkat, U-251MG, A549, MCF-7, and HEK293, as well as mouse and rat brain tissue lysates, confirming reliable cross-species reactivity. The observed band at approximately 50 kDa, slightly higher than the predicted 46 kDa molecular weight, likely reflects post-translational modifications characteristic of this protein. Immunofluorescence studies in HeLa cells reveal clear cytoplasmic and nuclear localization patterns, while flow cytometry validation in SH-SY5Y neuroblastoma cells demonstrates suitability for quantitative single-cell analysis.

This antibody serves researchers investigating proteasome biology, protein homeostasis, neurodegenerative disease mechanisms, and hormone receptor signaling pathways where PSMC5 function is implicated.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

Gene References into Functions

autoinflammation-associated H443P nlrc4 mutant is altered in interaction with SUG1 and ubiquitinated proteins, triggering constitutive caspase-8-mediated cell death dependent on FADD but independent of Ser(533) phosphorylation. PMID: 27974463
Our data suggest that PSMC5 facilitates the damaging effects of radiation in radiation-responsive H460 cancer cells and therefore may serve as a prognostic indicator for radiotherapy and molecular targeted therapy in lung cancer patients. PMID: 26592665
XopJ has protease activity to specifically degrade RPT6. PMID: 25739698
TRIP-1 regulates fibroblast acquisition of phenotype and function associated with myofibroblasts. PMID: 24528651
gamma-aminobutyric acidB receptor proteasomal degradation is mediated by the interaction of the GABAB2 C terminus with the proteasomal ATPase Rtp6 and regulated by neuronal activity PMID: 24482233
Rpt6 interacts directly with CKIP-1 and promotes the turnover of Smurf1. PMID: 23032291
Sug1 plays a critical role in transcription of MHC class I, and the MHC class II-like molecules, HLA-DM and HLA-DO. PMID: 22771340
Findings show that Sug1 is crucial for regulating histone H3K4me3 and H3R17me2 at the cytokine inducible MHC-II and CIITA promoters. PMID: 19660582
SUG1 has a role in ubiquitin/proteasome-mediated degradation of estrogen receptors PMID: 14702340
Proteasome dysfunction by a proteasome inhibitor or siRNA-mediated knock-down of Sug1 caused the up-regulation of MYO18B protein and MYO18B was polyubiquitinated in vivo. PMID: 16499872
p45 plays an important role in regulating ataxin-3 degradation by the proteasome. PMID: 17302910
These data demonstrate that both 19S and 20S subunits of the 26S proteasome play specific and critical roles in regulating CIITA activity and MHC class II transcription. PMID: 18215421
The current study strongly implicates the 19S ATPase Sug1 in modifying histones to initiate major histocompatibility complex class II transcription and provides novel insights into the role of the proteasome in the regulation of mammalian transcription. PMID: 18662994
SUG-1 has a unique role in linking the transcription and degradation processes via its ability to interact with SRC-3. PMID: 19144644

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

AAA ATPase family

Database Links

HGNC: 9552

OMIM: 601681

KEGG: hsa:5705

STRING: 9606.ENSP00000310572

UniGene: Hs.79387

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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