RNF7 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA776042A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: RNF7 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time：2min IHC image of CSB-RA776042A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA776042A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA776042A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9UBF6

Target Names

RNF7

Alternative Names

CKBBP 1 antibody; CKBBP1 antibody; CKII beta binding protein 1 antibody; CKII beta-binding protein 1 antibody; Rbx 2 antibody; Rbx2 antibody; RBX2_HUMAN antibody; Regulator of cullins 2 antibody; RING box protein 2 antibody; RING finger protein 7 antibody; RING-box protein 2 antibody; RNF 7 antibody; RNF7 antibody; ROC 2 antibody; ROC2 antibody; SAG antibody; Sensitive to apoptosis gene antibody; Sensitive to apoptosis gene protein antibody; Zinc RING finger protein SAG antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human RNF7 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

RNF7, also known as SAG (Sensitive to Apoptosis Gene) or ROC2, serves as a critical RING-box protein within cullin-RING E3 ubiquitin ligase complexes. This small but essential protein functions as a scaffold that recruits E2 ubiquitin-conjugating enzymes, thereby regulating the ubiquitination and subsequent degradation of numerous cellular substrates involved in cell cycle progression, apoptosis, and stress responses. Its role in modulating cellular sensitivity to apoptotic signals makes RNF7 a compelling target for researchers investigating cancer biology, protein homeostasis, and cell death pathways.

This recombinant monoclonal antibody, generated from clone 8C10 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. Affinity chromatography purification ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple platforms. Western blot analysis detects a clean band at the predicted 13 kDa molecular weight in HeLa, HepG2, COLO-205, and A431 cell lysates, confirming specificity across diverse human cancer cell lines. Immunohistochemistry staining has been optimized in paraffin-embedded human kidney and liver cancer tissues using citrate buffer antigen retrieval, while flow cytometry validation in 786-O renal carcinoma cells shows clear positive signal separation from isotype controls.

Whether you are investigating ubiquitin-proteasome pathway regulation, characterizing RNF7 expression patterns in tumor samples, or quantifying protein levels across experimental conditions, this antibody provides the flexibility to move seamlessly between detection methods in your cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

Gene References into Functions

We demonstrated that RNF7 knockdown induced growth suppression of prostate cancer cells and inactivated ERK1/2 pathway, which suggested RNF7 might be a potential novel therapeutic target for CRPC. PMID: 28252001
Results identified RNF7 to interact with CARMA2 regulating its NF-kappaB-activating capacity. Mechanistically, RNF7 influences CARMA2 signaling by regulating the ubiquitination state of MALT1 and the NF-kappaB-regulatory molecule NEMO. PMID: 29194363
Sag is a Kras-cooperating oncogene that promotes lung tumorigenesis PMID: 24430184
NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function. PMID: 25216516
RNF7 gene variant is associated with the risk of developing liver fibrosis and cirrhosis in an Eastern European population. PMID: 28338112
MAF1, RNF7 and SETD3 are identified as PCNA-associated proteins in human cells and given this interaction with PCNA, Maf1, RNF7, and SetD3 are potentially involved in DNA replication, DNA repair, or associated processes. PMID: 26030842
These findings indicate that Rbx1 and Rbx2 can both activate Cul5-Vif E3 ligase in vitro, but they may undergo a more delicate selection mechanism in vivo. PMID: 25912140
Single nucleotide polymorphism rs16851720 was associated with liver fibrosis progression. PMID: 22841784
Data suggest that the sensitive-to-apoptosis gene may be a candidate gene for good prognosis in rectal cancer, independent of therapeutic response of different individuals. PMID: 22171132
SAG plays an important role in regulating ionizing radiation-induced apoptosis PMID: 20933570
The findings showed that SAG E3 ubiquitin ligase plays an essential role in cancer cell proliferation and tumor growth PMID: 20103673
SAG possesses a potent peroxidase property to decompose hydrogen peroxide in the presence of dithiothreitol PMID: 11999705
results show that the Ring-H2 finger motif of CKBBP1 is necessary for efficient binding to CKIIbeta, as well as for optimal cell proliferation PMID: 12470599
sensitive to apoptosis gene protein inhibits peroxynitrite-induced DNA damage. PMID: 12565832
results indicate that protein kinase CKII may control IkappaBalpha and p27Kip1 degradation and thereby G1/S phase transition through the phosphorylation of threonine 10 within CKBBP1 protein PMID: 12748192
These studies suggested that CK2 might regulate SAG-SCF E3 ligase activity through modulating SAG's stability, rather than its enzymatic activity directly. PMID: 16874460
Endogenous levels of pro-caspase 3 were decreased by overexpression of SAG protein. PMID: 17217622
Promotes hypoxia-inducible factor 1 alpha subunit (HIF-1 alpha) ubiquitination and degradation. PMID: 17828303
Sensitive to apoptosis gene may play an important role in regulating the apoptosis induced by heat shock presumably through maintaining the cellular redox status. PMID: 18454945

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

RING-box family

Tissue Specificity

Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

Database Links

HGNC: 10070

OMIM: 603863

KEGG: hsa:9616

STRING: 9606.ENSP00000273480

UniGene: Hs.134623

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Ubiquitin mediated proteolysis

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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