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S1PR3 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA859932MA1HU
Size US$210
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Image
  • IHC image of CSB-RA859932MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Q99500
Target Names
S1PR3
Alternative Names
EDG 3 antibody; EDG3 antibody; Endothelial differentiation G protein coupled receptor 3 antibody; Endothelial differentiation G-protein coupled receptor 3 antibody; Endothelial differentiation gene 3 antibody; Endothelial differentiation sphingolipid G protein coupled receptor 3 antibody; FLJ37523 antibody; FLJ93220 antibody; G protein coupled receptor endothelial differentiation gene 3 antibody; LPB 3 antibody; LPB3 antibody; MGC71696 antibody; OTTHUMP00000215691 antibody; S1P receptor 3 antibody; S1P receptor Edg 3 antibody; S1P receptor Edg-3 antibody; S1P receptor EDG3 antibody; S1P3 antibody; S1pr3 antibody; S1PR3_HUMAN antibody; Sphingosine 1 phosphate receptor 3 antibody; Sphingosine 1 phosphate receptor Edg 3 antibody; Sphingosine 1 phosphate receptor Edg3 antibody; Sphingosine 1-phosphate receptor 3 antibody; Sphingosine 1-phosphate receptor Edg-3 antibody
Species Reactivity
Human
Immunogen
Recombinant Human S1PR3 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
28E10
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Protocols
ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Sphingosine 1-phosphate receptor 3 (S1PR3), also known as EDG3, is a G protein-coupled receptor that plays a central role in sphingolipid signaling pathways. This receptor mediates diverse cellular responses including vascular development, immune cell trafficking, and endothelial barrier function. S1PR3 signaling has garnered significant research attention for its involvement in cardiovascular physiology, inflammatory responses, and cancer progression, making it a compelling target for investigators studying lipid-mediated signal transduction.

This recombinant monoclonal antibody, clone 28E10, offers the reproducibility and consistency that demanding research protocols require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high specificity for your target while minimizing background interference.

Validation studies confirm reliable performance in immunohistochemistry applications, with successful staining demonstrated in paraffin-embedded human placenta tissue at dilutions ranging from 1:50 to 1:200. The IHC protocol employed citrate buffer antigen retrieval under high pressure conditions, with overnight primary antibody incubation at 4°C yielding clear visualization of S1PR3 expression patterns. This antibody is also validated for ELISA applications, providing flexibility across different experimental workflows.

Whether you are investigating sphingolipid receptor biology in vascular tissues, characterizing S1PR3 expression in pathological specimens, or exploring downstream signaling cascades, this recombinant antibody delivers the performance consistency essential for meaningful signal transduction research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Gene References into Functions
  1. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. PMID: 30006619
  2. Authors found that elevated levels of pSphK1 were positive correlation with high expression of S1P, which in turn promoted metastasis of TNBC through S1P/S1PR3/Notch signaling pathway. PMID: 29605826
  3. Sphingosine-1-phosphate receptor 3 (S1PR3) mediates multiple aspects of the inflammatory response during sepsis. PMID: 28850247
  4. in breast cancer cells overexpression of S1P3 and its activation by S1P has pro-inflammatory and pro-metastatic potential by inducing COX-2 expression and PGE2 signaling via EP2 and EP4. PMID: 27616330
  5. High S1PR3 expression is associated with increased lung adenocarcinoma. PMID: 27856637
  6. The study shows that S1P receptor 3 (S1PR3) mRNA is overexpressed in EBV-positive NPC patient-derived xenografts and a subset of primary NPC tissues, and that knockdown of S1PR3 suppressed the activation of AKT and the S1P-induced migration of NPC cells. PMID: 28240350
  7. S1pr3 promotes leukocyte rolling by mobilization of endothelial P-selectin. PMID: 25832730
  8. Stimulation with sphingosine-1-phosphate enhances cancer stem cells via S1PR3 and subsequent Notch1 activation. PMID: 25254944
  9. TRPC1 functions as a major regulator of S1P3 and VEGFR2 expression. PMID: 25971967
  10. Data indicate that sphingosine 1-phosphate (S1P) and hepatocyte growth factor (HGF) induced transloaction of integrin beta4, S1P receptors S1PR2 and S1PR3 to endothelial cell membrane caveolin-enriched microdomains (CEMs). PMID: 24851274
  11. a novel signaling axis, i.e., ROCK-JNK-ETS-1-CD44 pathway, which plays an essential role in the S1P3-regulated chemotactic response. PMID: 24064218
  12. associations were found for 2 promoter SNPs across 2 European descent samples supporting association of alleles -1899G and -1785C with decreased risk for sepsis-associated acute respiratory distress syndrome (ARDS); alleles reduced transcription factor binding to S1PR3 promoter and S1PR3 promoter activity; associated with lower plasma S1PR3 protein in ARDS patients PMID: 23911438
  13. Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis. PMID: 23918395
  14. S1PR agonists are pro-fibrotic via S1P2R and S1P3R stimulation using Smad-independent pathways. PMID: 23589284
  15. S1P3 receptors are detected in virtually all neurons in dorsal root ganglion and mediate pain behavior. PMID: 23392686
  16. Inhibition of S1P3 receptors prevented E2-induced activation of Cdc42, supporting the important role of the S1P receptor in E2 signaling. PMID: 23142484
  17. S1PR3 is nitrated on specific tyrosine residues and released as endothelial cell microparticles during acute lung injury. PMID: 22771388
  18. Suggest that the enhanced S1P3-EGFR signaling axis may contribute to the tumorigenesis or progression of lung adenocarcinomas. PMID: 22344462
  19. S1PR3 was higher in iliac-femoral arteries compared with carotid arteries. PMID: 22308044
  20. S1P regulates MMP-9 induction and invasiveness through coupling of S1P3 and G(alphaq) in MCF10A cells, thus providing a molecular basis for the crucial role of S1P in promoting breast cell invasion. PMID: 21652634
  21. These results suggest a potential role for HDL and S1P receptors in the pathogenesis of prostate cancer. PMID: 20979115
  22. The follicular fluid-HDL-associated S1P promotes granulosa lutein cell migration via S1PR3 and RAC1 activation. This may represent a novel mechanism contributing to the development of the corpus luteum. PMID: 20980685
  23. amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo. PMID: 19748727
  24. a 9-amino acid peptide (KRX-725), derived from the second intracellular loop of S1P3 (EDG3) mimics sphingosine 1-phosphate (S1P) by triggering a Gi-dependent mitogen-activated protein kinase kinase and extracellular signal-regulated kinase signal. PMID: 12763936
  25. S1P(3) function is not subject to conventional regulation by GRK phosphorylation PMID: 15894172
  26. Expression of the sphingosine 1 phosphate receptor in primary endothelial cells is both sufficient and necessary for the expression of Akt3. PMID: 16527273
  27. Reults describe the role of mu opioid receptors and S1P3 transactivation in the attenuation of vascular permeability by methylnaltrexone. PMID: 17395891
  28. Cytosolic phospholipase A2alpha activation induced by S1P is mediated by the S1P3 receptor in lung epithelial cells.( PMID: 18502815
  29. Results suggest that S1PR3 is involved in COX2 dependent effects of high-density lipoprotein on vascular smooth muscle. PMID: 18612546
  30. Report different response patterns of several ligands at the sphingosine-1-phosphate receptor subtype 3 (S1P(3)). PMID: 19309361

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Database Links

HGNC: 3167

OMIM: 601965

KEGG: hsa:1903

STRING: 9606.ENSP00000350878

UniGene: Hs.585118

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Related pathways
Sphingolipid signaling pathway G protein coupled receptor signaling pathway
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