S1PR3 Antibody

Code CSB-PA859932LA01HU
Size US$166
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Image
  • IHC image of CSB-PA859932LA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA859932LA01HU diluted at 1:800 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA859932LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) S1PR3 Polyclonal antibody
Uniprot No.
Target Names
S1PR3
Alternative Names
S1PR3; EDG3; Sphingosine 1-phosphate receptor 3; S1P receptor 3; S1P3; Endothelial differentiation G-protein coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3; S1P receptor Edg-3
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Sphingosine 1-phosphate receptor 3 protein (303-378AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The S1PR3 Antibody (Product code: CSB-PA859932LA01HU) is Non-conjugated. For S1PR3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA859932LB01HU S1PR3 Antibody, HRP conjugated ELISA
FITC CSB-PA859932LC01HU S1PR3 Antibody, FITC conjugated
Biotin CSB-PA859932LD01HU S1PR3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
IF 1:50-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Gene References into Functions
  1. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases. PMID: 30006619
  2. Authors found that elevated levels of pSphK1 were positive correlation with high expression of S1P, which in turn promoted metastasis of TNBC through S1P/S1PR3/Notch signaling pathway. PMID: 29605826
  3. Sphingosine-1-phosphate receptor 3 (S1PR3) mediates multiple aspects of the inflammatory response during sepsis. PMID: 28850247
  4. in breast cancer cells overexpression of S1P3 and its activation by S1P has pro-inflammatory and pro-metastatic potential by inducing COX-2 expression and PGE2 signaling via EP2 and EP4. PMID: 27616330
  5. High S1PR3 expression is associated with increased lung adenocarcinoma. PMID: 27856637
  6. The study shows that S1P receptor 3 (S1PR3) mRNA is overexpressed in EBV-positive NPC patient-derived xenografts and a subset of primary NPC tissues, and that knockdown of S1PR3 suppressed the activation of AKT and the S1P-induced migration of NPC cells. PMID: 28240350
  7. S1pr3 promotes leukocyte rolling by mobilization of endothelial P-selectin. PMID: 25832730
  8. Stimulation with sphingosine-1-phosphate enhances cancer stem cells via S1PR3 and subsequent Notch1 activation. PMID: 25254944
  9. TRPC1 functions as a major regulator of S1P3 and VEGFR2 expression. PMID: 25971967
  10. Data indicate that sphingosine 1-phosphate (S1P) and hepatocyte growth factor (HGF) induced transloaction of integrin beta4, S1P receptors S1PR2 and S1PR3 to endothelial cell membrane caveolin-enriched microdomains (CEMs). PMID: 24851274
  11. a novel signaling axis, i.e., ROCK-JNK-ETS-1-CD44 pathway, which plays an essential role in the S1P3-regulated chemotactic response. PMID: 24064218
  12. associations were found for 2 promoter SNPs across 2 European descent samples supporting association of alleles -1899G and -1785C with decreased risk for sepsis-associated acute respiratory distress syndrome (ARDS); alleles reduced transcription factor binding to S1PR3 promoter and S1PR3 promoter activity; associated with lower plasma S1PR3 protein in ARDS patients PMID: 23911438
  13. Sphingosine 1-phosphate receptor 3 regulates recruitment of anti-inflammatory monocytes to microvessels during implant arteriogenesis. PMID: 23918395
  14. S1PR agonists are pro-fibrotic via S1P2R and S1P3R stimulation using Smad-independent pathways. PMID: 23589284
  15. S1P3 receptors are detected in virtually all neurons in dorsal root ganglion and mediate pain behavior. PMID: 23392686
  16. Inhibition of S1P3 receptors prevented E2-induced activation of Cdc42, supporting the important role of the S1P receptor in E2 signaling. PMID: 23142484
  17. S1PR3 is nitrated on specific tyrosine residues and released as endothelial cell microparticles during acute lung injury. PMID: 22771388
  18. Suggest that the enhanced S1P3-EGFR signaling axis may contribute to the tumorigenesis or progression of lung adenocarcinomas. PMID: 22344462
  19. S1PR3 was higher in iliac-femoral arteries compared with carotid arteries. PMID: 22308044
  20. S1P regulates MMP-9 induction and invasiveness through coupling of S1P3 and G(alphaq) in MCF10A cells, thus providing a molecular basis for the crucial role of S1P in promoting breast cell invasion. PMID: 21652634
  21. These results suggest a potential role for HDL and S1P receptors in the pathogenesis of prostate cancer. PMID: 20979115
  22. The follicular fluid-HDL-associated S1P promotes granulosa lutein cell migration via S1PR3 and RAC1 activation. This may represent a novel mechanism contributing to the development of the corpus luteum. PMID: 20980685
  23. amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo. PMID: 19748727
  24. a 9-amino acid peptide (KRX-725), derived from the second intracellular loop of S1P3 (EDG3) mimics sphingosine 1-phosphate (S1P) by triggering a Gi-dependent mitogen-activated protein kinase kinase and extracellular signal-regulated kinase signal. PMID: 12763936
  25. S1P(3) function is not subject to conventional regulation by GRK phosphorylation PMID: 15894172
  26. Expression of the sphingosine 1 phosphate receptor in primary endothelial cells is both sufficient and necessary for the expression of Akt3. PMID: 16527273
  27. Reults describe the role of mu opioid receptors and S1P3 transactivation in the attenuation of vascular permeability by methylnaltrexone. PMID: 17395891
  28. Cytosolic phospholipase A2alpha activation induced by S1P is mediated by the S1P3 receptor in lung epithelial cells.( PMID: 18502815
  29. Results suggest that S1PR3 is involved in COX2 dependent effects of high-density lipoprotein on vascular smooth muscle. PMID: 18612546
  30. Report different response patterns of several ligands at the sphingosine-1-phosphate receptor subtype 3 (S1P(3)). PMID: 19309361

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Database Links

HGNC: 3167

OMIM: 601965

KEGG: hsa:1903

STRING: 9606.ENSP00000350878

UniGene: Hs.585118

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