Code | CSB-RA987662A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
FC | 1:20-1:200 |
The SDHB recombinant monoclonal antibody is produced through recombinant DNA technology and is ideal for detecting the SDHB protein from human, mouse, and rat samples in four applications including ELISA, WB, IHC, and FC. To produce this antibody, the cDNA of SDHB antibody-producing hybridomas is sequenced and the gene coding for the SDHB monoclonal antibody is synthesized. The hybridomas are formed by fusing myeloma cells B cells isolated from an animal that was immunized with a synthesized peptide derived from human SDHB. After cloning the synthesized gene into a vector, the recombinant vector is transfected into cells for cultivation. Finally, the resulting SDHB recombinant monoclonal antibody is purified from the cell culture supernatant through affinity chromatography.
The SDHB protein is a subunit of the succinate dehydrogenase (SDH) complex, which is an enzyme complex located in the inner mitochondrial membrane and plays a crucial role in the mitochondrial electron transport chain and the tricarboxylic acid (TCA) cycle. Specifically, SDHB acts as an anchor for the SDH complex and is involved in the electron transfer from succinate to ubiquinone during oxidative phosphorylation, leading to the generation of ATP. Mutations in the SDHB gene have been associated with several hereditary cancers, including paragangliomas, pheochromocytomas, and gastrointestinal stromal tumors.
There are currently no reviews for this product.